Time-resolved fluorescence measurements using self-pulsing 650-nm laser diodes

Daniel R. Matthews; Huw D. Summers; Kerenza Njoh; Rachel J. Errington; Paul J. Smith; Simon Ameer-Beg; Boris Vojnovic

doi:10.1117/12.590201

Time-resolved fluorescence measurements using self-pulsing 650-nm laser diodes

Daniel R. Matthews, Huw D. Summers, Kerenza Njoh, Rachel J. Errington, Paul J. Smith, Simon Ameer-Beg, Boris Vojnovic

Houston Methodist

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

We report on the development of a stroboscopic excitation technique using a self-pulsing laser, and show that it is a feasible method for obtaining fluorescence lifetime information from a biochip format. The self-pulsing lasers described here are versatile devices which have been used for one photon excitation measurements to determine the lifetime of cyanine 5 in water and ethanol. The same devices have been used to develop a technique whereby the emphasis for time-resolution of a lifetime measurement can be transferred to the excitation source from the detector and processing electronics by virtue of the multiple-pulse, variable frequency nature of the laser output.

Original language	English (US)
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Editors	A.N. Cartwright, M. Osinski
Pages	23-31
Number of pages	9
Volume	5705
DOIs	https://doi.org/10.1117/12.590201
State	Published - 2005
Event	Nanobiophotonics and Biomedical Applications II - San Jose, CA, United States Duration: Jan 24 2005 → Jan 27 2005

Other

Other	Nanobiophotonics and Biomedical Applications II
Country/Territory	United States
City	San Jose, CA
Period	1/24/05 → 1/27/05

ASJC Scopus subject areas

General Engineering

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10.1117/12.590201

Cite this

Matthews, DR, Summers, HD, Njoh, K, Errington, RJ, Smith, PJ, Ameer-Beg, S & Vojnovic, B 2005, Time-resolved fluorescence measurements using self-pulsing 650-nm laser diodes. in AN Cartwright & M Osinski (eds), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 5705, 06, pp. 23-31, Nanobiophotonics and Biomedical Applications II, San Jose, CA, United States, 1/24/05. https://doi.org/10.1117/12.590201

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title = "Time-resolved fluorescence measurements using self-pulsing 650-nm laser diodes",

abstract = "We report on the development of a stroboscopic excitation technique using a self-pulsing laser, and show that it is a feasible method for obtaining fluorescence lifetime information from a biochip format. The self-pulsing lasers described here are versatile devices which have been used for one photon excitation measurements to determine the lifetime of cyanine 5 in water and ethanol. The same devices have been used to develop a technique whereby the emphasis for time-resolution of a lifetime measurement can be transferred to the excitation source from the detector and processing electronics by virtue of the multiple-pulse, variable frequency nature of the laser output.",

author = "Matthews, \{Daniel R.\} and Summers, \{Huw D.\} and Kerenza Njoh and Errington, \{Rachel J.\} and Smith, \{Paul J.\} and Simon Ameer-Beg and Boris Vojnovic",

year = "2005",

doi = "10.1117/12.590201",

language = "English (US)",

volume = "5705",

pages = "23--31",

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AU - Matthews, Daniel R.

AU - Summers, Huw D.

AU - Njoh, Kerenza

AU - Errington, Rachel J.

AU - Smith, Paul J.

AU - Ameer-Beg, Simon

AU - Vojnovic, Boris

PY - 2005

Y1 - 2005

N2 - We report on the development of a stroboscopic excitation technique using a self-pulsing laser, and show that it is a feasible method for obtaining fluorescence lifetime information from a biochip format. The self-pulsing lasers described here are versatile devices which have been used for one photon excitation measurements to determine the lifetime of cyanine 5 in water and ethanol. The same devices have been used to develop a technique whereby the emphasis for time-resolution of a lifetime measurement can be transferred to the excitation source from the detector and processing electronics by virtue of the multiple-pulse, variable frequency nature of the laser output.

AB - We report on the development of a stroboscopic excitation technique using a self-pulsing laser, and show that it is a feasible method for obtaining fluorescence lifetime information from a biochip format. The self-pulsing lasers described here are versatile devices which have been used for one photon excitation measurements to determine the lifetime of cyanine 5 in water and ethanol. The same devices have been used to develop a technique whereby the emphasis for time-resolution of a lifetime measurement can be transferred to the excitation source from the detector and processing electronics by virtue of the multiple-pulse, variable frequency nature of the laser output.

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M3 - Conference contribution

AN - SCOPUS:23244445958

VL - 5705

SP - 23

EP - 31

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

A2 - Cartwright, A.N.

A2 - Osinski, M.

T2 - Nanobiophotonics and Biomedical Applications II

Y2 - 24 January 2005 through 27 January 2005

ER -

Time-resolved fluorescence measurements using self-pulsing 650-nm laser diodes

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