Time-resolved fluorescence measurements using self-pulsing 650-nm laser diodes

Daniel R. Matthews, Huw D. Summers, Kerenza Njoh, Rachel J. Errington, Paul J. Smith, Simon Ameer-Beg, Boris Vojnovic

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We report on the development of a stroboscopic excitation technique using a self-pulsing laser, and show that it is a feasible method for obtaining fluorescence lifetime information from a biochip format. The self-pulsing lasers described here are versatile devices which have been used for one photon excitation measurements to determine the lifetime of cyanine 5 in water and ethanol. The same devices have been used to develop a technique whereby the emphasis for time-resolution of a lifetime measurement can be transferred to the excitation source from the detector and processing electronics by virtue of the multiple-pulse, variable frequency nature of the laser output.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsA.N. Cartwright, M. Osinski
Pages23-31
Number of pages9
Volume5705
DOIs
StatePublished - 2005
EventNanobiophotonics and Biomedical Applications II - San Jose, CA, United States
Duration: Jan 24 2005Jan 27 2005

Other

OtherNanobiophotonics and Biomedical Applications II
CountryUnited States
CitySan Jose, CA
Period1/24/051/27/05

ASJC Scopus subject areas

  • Engineering(all)

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