Time dependent changes in aortic tissue during cold storage in physiological solution

M. G. Ghosn, M. Mashiatulla, M. A. Mohamed, S. Syed, F. Castro-Chavez, J. D. Morrisett, K. V. Larin

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Background: Stored vascular tissues are employed in biomedical research for studies in imaging, in biomechanics, and/or in assessing vessel diseases. In the present study, the stability of aortic tissue in phosphate buffer saline (PBS) at 4 °C was monitored over a course of 10 days as determined by the rate of glucose permeation measured by optical coherence tomography (OCT) and validated by histology. Methods and results: The initial mean permeability through fresh porcine aorta was (2.32 ± 0.46) × 10- 5 cm/s (n = 5); after maintaining the tissue at 4 °C for 10 days, the mean rate was (7.37 ± 0.41) × 10- 5 cm/s (n = 4), an increase of nearly 300%. A z-test verified that a significant change in the permeability rate (p < 0.05) had occurred after 4 days of 4 °C storage. Histology was used to quantify changes in tissue pore area. The increase in average pore area paralleled the increase in permeability rate over 10 days. Conclusions: These results suggest that (1) the structural integrity of aortic tissue at 4 °C is retained for at least the first three days after resection and (2) OCT is a powerful technology well suited for evaluating tissue structural integrity over time. General significance: Functional OCT imaging provides for a noninvasive and quantitative technique in determining the structural integrity of aortic tissue stored at 4 °C. This modality may be used for assessing the efficacy of other preservation techniques.

Original languageEnglish (US)
Pages (from-to)555-560
Number of pages6
JournalBiochimica et Biophysica Acta - General Subjects
Issue number5
StatePublished - May 2011


  • Cold tissue storage
  • Hypothermal preservation
  • Permeability rate
  • Porcine aorta
  • Pore size

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology


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