Thyroid hormone transport is disturbed in erythrocytes from patients with chronic renal failure on hemodialysis

Maria C.S. Rodrigues; Guilherme M. Santos; Aluízio Da C. Silva; John D. Baxter; Paul Webb; Noureddine Lomri; Francisco A.R. Neves; Ralff C.J. Ribeiro; Luiz A. Simeoni

doi:10.1081/JDI-200026760

Thyroid hormone transport is disturbed in erythrocytes from patients with chronic renal failure on hemodialysis

Maria C.S. Rodrigues, Guilherme M. Santos, Aluízio Da C. Silva, John D. Baxter, Paul Webb, Noureddine Lomri, Francisco A.R. Neves, Ralff C.J. Ribeiro, Luiz A. Simeoni

Academic Institute

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Aims: To now, there are no studies reporting whether thyroid hormones (THs) transport play a role in thyroid hormone dysfunction observed in chronic renal failure (CRF). Therefore, the aim of this study was to investigate the transport of THs in erythrocytes frem patients with CRF on hemodialysis (HD). Methods: [¹²⁵I-L-triiodothyronine ([¹²⁵I]T₃) and ([ ¹²⁵I]-L-thyroxine ([¹²⁵I]T₄) erythrocytes uptake was measured at 1 min and 5 min. To study L-triiodothyronine (LT ₃) and L-thyroxine (LT₄) efflux from erythrocytes, we preloaded the cells during 180 min with [¹²⁵I]T₃ or [ ¹²⁵I]T₄ and measured their [¹²⁵I]T₃ or [¹²⁵I]T₄ efflux during 60 min. Results: [ ¹²⁵I]T₃ uptake in erythrocytes from uremic patients pre-HD was higher than control subjects by 50% at 1 min and by 55% at 5 min. However, [¹²⁵I]T₄ uptake in erythrocytes from uremic patients was significantly lower at 1 min (88%) and at 5 min (63%). LT₃ efflux rate was lower and LT₄ efflux was significantly higher than in control subjects. After 60-min of efflux, LT³ remained in erythrocytes was 80% higher and LT₄ was 57% lower than in normal individuals. Neither [¹²⁵I]T₃ and [¹²⁵I]T ₄ uptake, nor efflux rates were changed by hemodialysis. Conclusion: Despite the fact that uremic patients on hemodialysis show low serum levels of LT₃. changes in LT₃ influx and efflux could act as a compensatory mechanism that neutralize thyroid hormone dysfunction in order to maintain the euthyroid state.

Original language	English (US)
Pages (from-to)	461-466
Number of pages	6
Journal	Renal Failure
Volume	26
Issue number	4
DOIs	https://doi.org/10.1081/JDI-200026760
State	Published - 2004

Keywords

Chronic renal failure
Human erythrocytes
Thyroid function
Thyroid hormones transport

ASJC Scopus subject areas

Nephrology

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10.1081/JDI-200026760

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@article{0f68165e3a704217b118470562f31f00,

title = "Thyroid hormone transport is disturbed in erythrocytes from patients with chronic renal failure on hemodialysis",

abstract = "Aims: To now, there are no studies reporting whether thyroid hormones (THs) transport play a role in thyroid hormone dysfunction observed in chronic renal failure (CRF). Therefore, the aim of this study was to investigate the transport of THs in erythrocytes frem patients with CRF on hemodialysis (HD). Methods: [125I-L-triiodothyronine ([125I]T3) and ([ 125I]-L-thyroxine ([125I]T4) erythrocytes uptake was measured at 1 min and 5 min. To study L-triiodothyronine (LT 3) and L-thyroxine (LT4) efflux from erythrocytes, we preloaded the cells during 180 min with [125I]T3 or [ 125I]T4 and measured their [125I]T3 or [125I]T4 efflux during 60 min. Results: [ 125I]T3 uptake in erythrocytes from uremic patients pre-HD was higher than control subjects by 50% at 1 min and by 55% at 5 min. However, [125I]T4 uptake in erythrocytes from uremic patients was significantly lower at 1 min (88%) and at 5 min (63%). LT3 efflux rate was lower and LT4 efflux was significantly higher than in control subjects. After 60-min of efflux, LT3 remained in erythrocytes was 80% higher and LT4 was 57% lower than in normal individuals. Neither [125I]T3 and [125I]T 4 uptake, nor efflux rates were changed by hemodialysis. Conclusion: Despite the fact that uremic patients on hemodialysis show low serum levels of LT3. changes in LT3 influx and efflux could act as a compensatory mechanism that neutralize thyroid hormone dysfunction in order to maintain the euthyroid state.",

keywords = "Chronic renal failure, Human erythrocytes, Thyroid function, Thyroid hormones transport",

author = "Rodrigues, {Maria C.S.} and Santos, {Guilherme M.} and Silva, {Alu{\'i}zio Da C.} and Baxter, {John D.} and Paul Webb and Noureddine Lomri and Neves, {Francisco A.R.} and Ribeiro, {Ralff C.J.} and Simeoni, {Luiz A.}",

note = "Funding Information: The Brazilian Research Council, CNPq, grant 471204/01-3 to L.A.S. and CAPES-COFECUB Program grant 434/03 to G.M.S., F.A.R.N., N.L. and L.A.S. supported this work. The authors wish to thank Soclimed Dialysis Unit{\textquoteright}s staff, Mrs. Rilva G. P. Soares for technical assistance and Mrs. Evelyne Ribeiro for her assistance with the English manuscript.",

year = "2004",

doi = "10.1081/JDI-200026760",

language = "English (US)",

volume = "26",

pages = "461--466",

journal = "Renal Failure",

issn = "0886-022X",

publisher = "Informa Healthcare",

number = "4",

}

TY - JOUR

T1 - Thyroid hormone transport is disturbed in erythrocytes from patients with chronic renal failure on hemodialysis

AU - Rodrigues, Maria C.S.

AU - Santos, Guilherme M.

AU - Silva, Aluízio Da C.

AU - Baxter, John D.

AU - Webb, Paul

AU - Lomri, Noureddine

AU - Neves, Francisco A.R.

AU - Ribeiro, Ralff C.J.

AU - Simeoni, Luiz A.

N1 - Funding Information: The Brazilian Research Council, CNPq, grant 471204/01-3 to L.A.S. and CAPES-COFECUB Program grant 434/03 to G.M.S., F.A.R.N., N.L. and L.A.S. supported this work. The authors wish to thank Soclimed Dialysis Unit’s staff, Mrs. Rilva G. P. Soares for technical assistance and Mrs. Evelyne Ribeiro for her assistance with the English manuscript.

PY - 2004

Y1 - 2004

N2 - Aims: To now, there are no studies reporting whether thyroid hormones (THs) transport play a role in thyroid hormone dysfunction observed in chronic renal failure (CRF). Therefore, the aim of this study was to investigate the transport of THs in erythrocytes frem patients with CRF on hemodialysis (HD). Methods: [125I-L-triiodothyronine ([125I]T3) and ([ 125I]-L-thyroxine ([125I]T4) erythrocytes uptake was measured at 1 min and 5 min. To study L-triiodothyronine (LT 3) and L-thyroxine (LT4) efflux from erythrocytes, we preloaded the cells during 180 min with [125I]T3 or [ 125I]T4 and measured their [125I]T3 or [125I]T4 efflux during 60 min. Results: [ 125I]T3 uptake in erythrocytes from uremic patients pre-HD was higher than control subjects by 50% at 1 min and by 55% at 5 min. However, [125I]T4 uptake in erythrocytes from uremic patients was significantly lower at 1 min (88%) and at 5 min (63%). LT3 efflux rate was lower and LT4 efflux was significantly higher than in control subjects. After 60-min of efflux, LT3 remained in erythrocytes was 80% higher and LT4 was 57% lower than in normal individuals. Neither [125I]T3 and [125I]T 4 uptake, nor efflux rates were changed by hemodialysis. Conclusion: Despite the fact that uremic patients on hemodialysis show low serum levels of LT3. changes in LT3 influx and efflux could act as a compensatory mechanism that neutralize thyroid hormone dysfunction in order to maintain the euthyroid state.

AB - Aims: To now, there are no studies reporting whether thyroid hormones (THs) transport play a role in thyroid hormone dysfunction observed in chronic renal failure (CRF). Therefore, the aim of this study was to investigate the transport of THs in erythrocytes frem patients with CRF on hemodialysis (HD). Methods: [125I-L-triiodothyronine ([125I]T3) and ([ 125I]-L-thyroxine ([125I]T4) erythrocytes uptake was measured at 1 min and 5 min. To study L-triiodothyronine (LT 3) and L-thyroxine (LT4) efflux from erythrocytes, we preloaded the cells during 180 min with [125I]T3 or [ 125I]T4 and measured their [125I]T3 or [125I]T4 efflux during 60 min. Results: [ 125I]T3 uptake in erythrocytes from uremic patients pre-HD was higher than control subjects by 50% at 1 min and by 55% at 5 min. However, [125I]T4 uptake in erythrocytes from uremic patients was significantly lower at 1 min (88%) and at 5 min (63%). LT3 efflux rate was lower and LT4 efflux was significantly higher than in control subjects. After 60-min of efflux, LT3 remained in erythrocytes was 80% higher and LT4 was 57% lower than in normal individuals. Neither [125I]T3 and [125I]T 4 uptake, nor efflux rates were changed by hemodialysis. Conclusion: Despite the fact that uremic patients on hemodialysis show low serum levels of LT3. changes in LT3 influx and efflux could act as a compensatory mechanism that neutralize thyroid hormone dysfunction in order to maintain the euthyroid state.

KW - Chronic renal failure

KW - Human erythrocytes

KW - Thyroid function

KW - Thyroid hormones transport

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U2 - 10.1081/JDI-200026760

DO - 10.1081/JDI-200026760

M3 - Article

C2 - 15462116

AN - SCOPUS:4444298892

SN - 0886-022X

VL - 26

SP - 461

EP - 466

JO - Renal Failure

JF - Renal Failure

IS - 4

ER -

Thyroid hormone transport is disturbed in erythrocytes from patients with chronic renal failure on hemodialysis

Abstract

Keywords

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