Three-dimensional printed polymeric system to encapsulate human mesenchymal stem cells differentiated into islet-like insulin-producing aggregates for diabetes treatment

Omaima Sabek, Marco Farina, Daniel W. Fraga, Solmaz Afshar, Andrea Ballerini, Carly S. Filgueira, Usha R. Thekkedath, Alessandro Grattoni, A. Osama Gaber

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Diabetes is one of the most prevalent, costly, and debilitating diseases in the world. Pancreas and islet transplants have shown success in re-establishing glucose control and reversing diabetic complications. However, both are limited by donor availability, need for continuous immunosuppression, loss of transplanted tissue due to dispersion, and lack of vascularization. To overcome the limitations of poor islet availability, here, we investigate the potential of bone marrow-derived mesenchymal stem cells differentiated into islet-like insulin-producing aggregates. Islet-like insulin-producing aggregates, characterized by gene expression, are shown to be similar to pancreatic islets and display positive immunostaining for insulin and glucagon. To address the limits of current encapsulation systems, we developed a novel three-dimensional printed, scalable, and potentially refillable polymeric construct (nanogland) to support islet-like insulin-producing aggregates' survival and function in the host body. In vitro studies showed that encapsulated islet-like insulin-producing aggregates maintained viability and function, producing steady levels of insulin for at least 4 weeks. Nanogland-islet-like insulin-producing aggregate technology here investigated as a proof of concept holds potential as an effective and innovative approach for diabetes cell therapy.

Original languageEnglish (US)
Number of pages13
JournalJournal of Tissue Engineering
Volume7
Early online dateApr 22 2016
DOIs
StatePublished - Apr 22 2016

Keywords

  • diabetes
  • Human bone marrow
  • mesenchymal stem cells
  • polylactic acid
  • scaffold
  • three-dimensional printer

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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