Abstract
DNA-DNA reactions can be monitored with a label-free fluorogenic reaction. Guanosine-rich, single-stranded DNA oligonucleotides bind to thioflavin-T (ThT) and enhance the fluorescence of the dye. We discovered a novel DNA sequence that produces fluorescence upon binding to ThT. We denote this oligonucleotide ThTSignal. We use ThTSignal as a label-free reporter for the activity of several designed DNA-DNA reactions (DNA circuits). The DNA circuits conditionally produce the ThTSignal oligonucleotide by association or by liberating the ThTSignal oligonucleotide from double-stranded DNA. This strategy offers label-free, cost-effective, fluorogenic detection of the molecular beacon reaction, split reporter reaction, one-step strand displacement reaction, and the entropy-driven amplifier reaction (a catalytic DNA circuit).
Original language | English (US) |
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Article number | 045001 |
Journal | Methods and Applications in Fluorescence |
Volume | 8 |
Issue number | 4 |
DOIs | |
State | Published - Oct 2020 |
Keywords
- G-rich sequence
- Thioflavin-T
- entropy-driven amplifier reaction
- label-freeDNAcircuits
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Instrumentation
- Materials Science(all)
- Spectroscopy