The yeast α-factor receptor: Structural properties deduced from the sequence of the STE2 gene

Anne C. Burkholder; Leland H. Hartwell

doi:10.1093/nar/13.23.8463

The yeast α-factor receptor: Structural properties deduced from the sequence of the STE2 gene

Anne C. Burkholder, Leland H. Hartwell

Houston Methodist

Research output: Contribution to journal › Article › peer-review

227 Scopus citations

Abstract

The STE2 gene of the yeast Saccharomyces cerevisiae encodes a component of the receptor for the oligopeptide pheromone α-factor (1). We have cloned and determined the nucleotide sequence of the STE2 gene. A sequence involved in the control of cell-type expression of the STE2 gene (2,3) was found 5′ of an open reading frame that could encode a protein of 431 amino acids. The predicted STE2 protein contains seven hydrophobic segments, suggesting that the α-factor receptor is an integral membrane protein. No extensive homology at the primary sequence level was detected between the predicted STE2 protein and other available protein sequences.

Original language	English (US)
Pages (from-to)	8463-8475
Number of pages	13
Journal	Nucleic Acids Research
Volume	13
Issue number	23
DOIs	https://doi.org/10.1093/nar/13.23.8463
State	Published - Dec 9 1985

ASJC Scopus subject areas

Genetics

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title = "The yeast α-factor receptor: Structural properties deduced from the sequence of the STE2 gene",

abstract = "The STE2 gene of the yeast Saccharomyces cerevisiae encodes a component of the receptor for the oligopeptide pheromone α-factor (1). We have cloned and determined the nucleotide sequence of the STE2 gene. A sequence involved in the control of cell-type expression of the STE2 gene (2,3) was found 5′ of an open reading frame that could encode a protein of 431 amino acids. The predicted STE2 protein contains seven hydrophobic segments, suggesting that the α-factor receptor is an integral membrane protein. No extensive homology at the primary sequence level was detected between the predicted STE2 protein and other available protein sequences.",

author = "Burkholder, \{Anne C.\} and Hartwell, \{Leland H.\}",

note = "Funding Information: ACKNOWLEDGEMENTS We thank. C. Furlong and R. Garber for advice on DNA sequencing, J. Brown and S. Eiaenberg for assistance with the computer analysis, H. Fried for contributing the yeast genomic clone bank, D. Jenness for his gift of radioactive a-factor, and D. Hagen, G. Sprague, A. Johnson, K. Wilson, and I. Herskowitz for communication of results prior to publication. We also thank D. Burke, D. Koshland, and J. Konopka for their comments on the manuscript. This investigation was supported by NIH research grant number GM177O9 from the Institute of General Medical Sciences and a grant from the American Business Research Foundation. A.C.B. was supported by Public Health Service Training Grant 5T32GM07735.",

year = "1985",

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doi = "10.1093/nar/13.23.8463",

language = "English (US)",

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T2 - Structural properties deduced from the sequence of the STE2 gene

AU - Burkholder, Anne C.

AU - Hartwell, Leland H.

N1 - Funding Information: ACKNOWLEDGEMENTS We thank. C. Furlong and R. Garber for advice on DNA sequencing, J. Brown and S. Eiaenberg for assistance with the computer analysis, H. Fried for contributing the yeast genomic clone bank, D. Jenness for his gift of radioactive a-factor, and D. Hagen, G. Sprague, A. Johnson, K. Wilson, and I. Herskowitz for communication of results prior to publication. We also thank D. Burke, D. Koshland, and J. Konopka for their comments on the manuscript. This investigation was supported by NIH research grant number GM177O9 from the Institute of General Medical Sciences and a grant from the American Business Research Foundation. A.C.B. was supported by Public Health Service Training Grant 5T32GM07735.

PY - 1985/12/9

Y1 - 1985/12/9

N2 - The STE2 gene of the yeast Saccharomyces cerevisiae encodes a component of the receptor for the oligopeptide pheromone α-factor (1). We have cloned and determined the nucleotide sequence of the STE2 gene. A sequence involved in the control of cell-type expression of the STE2 gene (2,3) was found 5′ of an open reading frame that could encode a protein of 431 amino acids. The predicted STE2 protein contains seven hydrophobic segments, suggesting that the α-factor receptor is an integral membrane protein. No extensive homology at the primary sequence level was detected between the predicted STE2 protein and other available protein sequences.

AB - The STE2 gene of the yeast Saccharomyces cerevisiae encodes a component of the receptor for the oligopeptide pheromone α-factor (1). We have cloned and determined the nucleotide sequence of the STE2 gene. A sequence involved in the control of cell-type expression of the STE2 gene (2,3) was found 5′ of an open reading frame that could encode a protein of 431 amino acids. The predicted STE2 protein contains seven hydrophobic segments, suggesting that the α-factor receptor is an integral membrane protein. No extensive homology at the primary sequence level was detected between the predicted STE2 protein and other available protein sequences.

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ER -

The yeast α-factor receptor: Structural properties deduced from the sequence of the STE2 gene

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