Abstract
E-cadherin junctions facilitate assembly and disassembly of cell contacts that drive development and homeostasis of epithelial tissues. In this study, using Xenopus embryonic kidney and Madin-Darby canine kidney (MDCK) cells, we investigate the role of the Wnt/planar cell polarity (PCP) formin Daam1 (Dishevelled-associated activator of morphogenesis 1) in regulating E-cadherin-based intercellular adhesion. Using live imaging, we show that Daam1 localizes to newly formed cell contacts in the developing nephron. Furthermore, analyses of junctional filamentous actin (F-actin) upon Daam1 depletion indicate decreased microfilament localization and slowed turnover. We also show that Daam1 is necessary for efficient and timely localization of junctional E-cadherin, mediated by Daam1’s formin homology domain 2 (FH2). Finally, we establish that Daam1 signaling promotes organized movement of renal cells. This study demonstrates that Daam1 formin junctional activity is critical for epithelial tissue organization.
Original language | English (US) |
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Article number | 109340 |
Journal | Cell Reports |
Volume | 36 |
Issue number | 1 |
DOIs | |
State | Published - Jul 6 2021 |
Keywords
- Daam1
- E-cadherin
- F-actin
- Wnt
- Xenopus
- adhesion
- formin
- kidney
- nephron
- tubulogenesis
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology