The viability of U-2 OS cells on Zinc Oxide nanowires observed via MTS assay in vitro

Richard A. Brown; Chris J. Barnett; Afshin Tarat; Dan R. Jones; Lewis W. Francis; Steven Conlan; Thierry G.G. Maffeis

doi:10.1109/NANO.2012.6322020

The viability of U-2 OS cells on Zinc Oxide nanowires observed via MTS assay in vitro

Richard A. Brown, Chris J. Barnett, Afshin Tarat, Dan R. Jones, Lewis W. Francis, Steven Conlan, Thierry G.G. Maffeis

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Scopus citations

Abstract

U-2 OS, an Osteosarcoma cell line was incubated on Zinc Oxide nanowire (ZnO NW) arrays of differing morphology for 72 hours at different seeding densities. MTS assays were conducted to ascertain the viability of the cells and the biocompatibility of ZnO NWs. It was found that the viability of the U-2 OS cell line when subjected to a high concentration of 1.22μg/ml NWs lies between 71-96% and 68-72% for substrates with a precursor chemical ratio of 21 and 11, respectively. It was observed that the shape of the NWs determined the viability of cells on the NWs; which was attributed to changes in the surface area available for cell adhesion. It was determined that the increased surface area of the 21 NW array provides a higher area for interaction and formation of focal adhesions compared to arrays formed from the smaller, pointier 11 NW array that inhibit cell proliferation due to non adhesive gaps.

Original language	English (US)
Title of host publication	2012 12th IEEE International Conference on Nanotechnology, NANO 2012
DOIs	https://doi.org/10.1109/NANO.2012.6322020
State	Published - 2012
Event	2012 12th IEEE International Conference on Nanotechnology, NANO 2012 - Birmingham, United Kingdom Duration: Aug 20 2012 → Aug 23 2012

Publication series

Name	Proceedings of the IEEE Conference on Nanotechnology
ISSN (Print)	1944-9399
ISSN (Electronic)	1944-9380

Other

Other	2012 12th IEEE International Conference on Nanotechnology, NANO 2012
Country/Territory	United Kingdom
City	Birmingham
Period	8/20/12 → 8/23/12

Keywords

Biocompatibility
Hydrothermal
MTS
Zinc Oxide

ASJC Scopus subject areas

Bioengineering
Electrical and Electronic Engineering
Materials Chemistry
Condensed Matter Physics

Access to Document

10.1109/NANO.2012.6322020

Cite this

Brown, R. A., Barnett, C. J., Tarat, A., Jones, D. R., Francis, L. W., Conlan, S., & Maffeis, T. G. G. (2012). The viability of U-2 OS cells on Zinc Oxide nanowires observed via MTS assay in vitro. In 2012 12th IEEE International Conference on Nanotechnology, NANO 2012 Article 6322020 (Proceedings of the IEEE Conference on Nanotechnology). https://doi.org/10.1109/NANO.2012.6322020

The viability of U-2 OS cells on Zinc Oxide nanowires observed via MTS assay in vitro. / Brown, Richard A.; Barnett, Chris J.; Tarat, Afshin et al.
2012 12th IEEE International Conference on Nanotechnology, NANO 2012. 2012. 6322020 (Proceedings of the IEEE Conference on Nanotechnology).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Brown, RA, Barnett, CJ, Tarat, A, Jones, DR, Francis, LW , Conlan, S & Maffeis, TGG 2012, The viability of U-2 OS cells on Zinc Oxide nanowires observed via MTS assay in vitro. in 2012 12th IEEE International Conference on Nanotechnology, NANO 2012., 6322020, Proceedings of the IEEE Conference on Nanotechnology, 2012 12th IEEE International Conference on Nanotechnology, NANO 2012, Birmingham, United Kingdom, 8/20/12. https://doi.org/10.1109/NANO.2012.6322020

@inproceedings{389eb633f3cf4a4a953c310016fadd17,

title = "The viability of U-2 OS cells on Zinc Oxide nanowires observed via MTS assay in vitro",

abstract = "U-2 OS, an Osteosarcoma cell line was incubated on Zinc Oxide nanowire (ZnO NW) arrays of differing morphology for 72 hours at different seeding densities. MTS assays were conducted to ascertain the viability of the cells and the biocompatibility of ZnO NWs. It was found that the viability of the U-2 OS cell line when subjected to a high concentration of 1.22μg/ml NWs lies between 71-96% and 68-72% for substrates with a precursor chemical ratio of 21 and 11, respectively. It was observed that the shape of the NWs determined the viability of cells on the NWs; which was attributed to changes in the surface area available for cell adhesion. It was determined that the increased surface area of the 21 NW array provides a higher area for interaction and formation of focal adhesions compared to arrays formed from the smaller, pointier 11 NW array that inhibit cell proliferation due to non adhesive gaps.",

keywords = "Biocompatibility, Hydrothermal, MTS, Zinc Oxide",

author = "Brown, {Richard A.} and Barnett, {Chris J.} and Afshin Tarat and Jones, {Dan R.} and Francis, {Lewis W.} and Steven Conlan and Maffeis, {Thierry G.G.}",

year = "2012",

doi = "10.1109/NANO.2012.6322020",

language = "English (US)",

isbn = "9781467321983",

series = "Proceedings of the IEEE Conference on Nanotechnology",

booktitle = "2012 12th IEEE International Conference on Nanotechnology, NANO 2012",

}

TY - GEN

T1 - The viability of U-2 OS cells on Zinc Oxide nanowires observed via MTS assay in vitro

AU - Brown, Richard A.

AU - Barnett, Chris J.

AU - Tarat, Afshin

AU - Jones, Dan R.

AU - Francis, Lewis W.

AU - Conlan, Steven

AU - Maffeis, Thierry G.G.

PY - 2012

Y1 - 2012

N2 - U-2 OS, an Osteosarcoma cell line was incubated on Zinc Oxide nanowire (ZnO NW) arrays of differing morphology for 72 hours at different seeding densities. MTS assays were conducted to ascertain the viability of the cells and the biocompatibility of ZnO NWs. It was found that the viability of the U-2 OS cell line when subjected to a high concentration of 1.22μg/ml NWs lies between 71-96% and 68-72% for substrates with a precursor chemical ratio of 21 and 11, respectively. It was observed that the shape of the NWs determined the viability of cells on the NWs; which was attributed to changes in the surface area available for cell adhesion. It was determined that the increased surface area of the 21 NW array provides a higher area for interaction and formation of focal adhesions compared to arrays formed from the smaller, pointier 11 NW array that inhibit cell proliferation due to non adhesive gaps.

AB - U-2 OS, an Osteosarcoma cell line was incubated on Zinc Oxide nanowire (ZnO NW) arrays of differing morphology for 72 hours at different seeding densities. MTS assays were conducted to ascertain the viability of the cells and the biocompatibility of ZnO NWs. It was found that the viability of the U-2 OS cell line when subjected to a high concentration of 1.22μg/ml NWs lies between 71-96% and 68-72% for substrates with a precursor chemical ratio of 21 and 11, respectively. It was observed that the shape of the NWs determined the viability of cells on the NWs; which was attributed to changes in the surface area available for cell adhesion. It was determined that the increased surface area of the 21 NW array provides a higher area for interaction and formation of focal adhesions compared to arrays formed from the smaller, pointier 11 NW array that inhibit cell proliferation due to non adhesive gaps.

KW - Biocompatibility

KW - Hydrothermal

KW - MTS

KW - Zinc Oxide

UR - http://www.scopus.com/inward/record.url?scp=84869149960&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84869149960&partnerID=8YFLogxK

U2 - 10.1109/NANO.2012.6322020

DO - 10.1109/NANO.2012.6322020

M3 - Conference contribution

AN - SCOPUS:84869149960

SN - 9781467321983

T3 - Proceedings of the IEEE Conference on Nanotechnology

BT - 2012 12th IEEE International Conference on Nanotechnology, NANO 2012

T2 - 2012 12th IEEE International Conference on Nanotechnology, NANO 2012

Y2 - 20 August 2012 through 23 August 2012

ER -

The viability of U-2 OS cells on Zinc Oxide nanowires observed via MTS assay in vitro

Abstract

Publication series

Other

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this