The viability of U-2 OS cells on Zinc Oxide nanowires observed via MTS assay in vitro

Richard A. Brown, Chris J. Barnett, Afshin Tarat, Dan R. Jones, Lewis W. Francis, R. Steven Conlan, Thierry G.G. Maffeis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

U-2 OS, an Osteosarcoma cell line was incubated on Zinc Oxide nanowire (ZnO NW) arrays of differing morphology for 72 hours at different seeding densities. MTS assays were conducted to ascertain the viability of the cells and the biocompatibility of ZnO NWs. It was found that the viability of the U-2 OS cell line when subjected to a high concentration of 1.22μg/ml NWs lies between 71-96% and 68-72% for substrates with a precursor chemical ratio of 21 and 11, respectively. It was observed that the shape of the NWs determined the viability of cells on the NWs; which was attributed to changes in the surface area available for cell adhesion. It was determined that the increased surface area of the 21 NW array provides a higher area for interaction and formation of focal adhesions compared to arrays formed from the smaller, pointier 11 NW array that inhibit cell proliferation due to non adhesive gaps.

Original languageEnglish (US)
Title of host publication2012 12th IEEE International Conference on Nanotechnology, NANO 2012
DOIs
StatePublished - Nov 22 2012
Event2012 12th IEEE International Conference on Nanotechnology, NANO 2012 - Birmingham, United Kingdom
Duration: Aug 20 2012Aug 23 2012

Other

Other2012 12th IEEE International Conference on Nanotechnology, NANO 2012
CountryUnited Kingdom
CityBirmingham
Period8/20/128/23/12

Keywords

  • Biocompatibility
  • Hydrothermal
  • MTS
  • Zinc Oxide

ASJC Scopus subject areas

  • Bioengineering
  • Electrical and Electronic Engineering
  • Materials Chemistry
  • Condensed Matter Physics

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