The two-component system GrvRS (EtaRS) regulates ace expression in enterococcus faecalis OG1RF

Jung Hyeob Roh, Kavindra V. Singh, Sabina Leanti La Rosa, Ana Luisa V. Cohen, Barbara E. Murray

    Research output: Contribution to journalArticlepeer-review

    11 Scopus citations


    Expression of ace (adhesin to collagen of Enterococcus faecalis), encoding a virulence factor in endocarditis and urinary tract infection models, has been shown to increase under certain conditions, such as in the presence of serum, bile salts, urine, and collagen and at 46°C. However, the mechanism of ace/Ace regulation under different conditions is still unknown. In this study, we identified a two-component regulatory system GrvRS as the main regulator of ace expression under these stress conditions. Using Northern hybridization and β-galactosidase assays of an ace promoter-lacZ fusion, we found transcription of ace to be virtually absent in a grvR deletion mutant under the conditions that increase ace expression in wild-type OG1RF and in the complemented strain. Moreover, a grvR mutant revealed decreased collagen binding and biofilm formation as well as attenuation in a murine urinary tract infection model. Here we show that GrvR plays a major role in control of ace expression and E. faecalis virulence.

    Original languageEnglish (US)
    Pages (from-to)389-395
    Number of pages7
    JournalInfection and Immunity
    Issue number1
    StatePublished - 2015

    ASJC Scopus subject areas

    • Parasitology
    • Microbiology
    • Immunology
    • Infectious Diseases


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