The translocation of fullerenic nanoparticles into lysosome via the pathway of clathrin-mediated endocytosis

Wei Li, Chunying Chen, Chang Ye, Taotao Wei, Yuliang Zhao, Fang Lao, Zhen Chen, Huan Meng, Yuxi Gao, Hui Yuan, Genmei Xing, Feng Zhao, Zhifang Chai, Xujia Zhang, Fuyu Yang, Dong Han, Xianhua Tang, Yingge Zhang

Research output: Contribution to journalArticlepeer-review

99 Scopus citations

Abstract

Manufactured fullerene nanoparticles easily enter into cells and hence have been rapidly developed for biomedical uses. However, it is generally unknown which route the nanoparticles undergo when crossing cell membranes and where they localize to the intracellular compartments. Herein we have used both microscopic imaging and biological techniques to explore the processes of [C60(C(COOH)2)2]n nanoparticles across cellular membranes and their intracellular translocation in 3T3 L1 and RH-35 living cells. The fullerene nanoparticles are quickly internalized by the cells and then routed to the cytoplasm with punctate localization. Upon entering the cell, they are synchronized to lysosome-like vesicles. The [C 60(C(COOH)2)2]n nanoparticles entering cells are mainly via endocytosis with time-, temperature- and energy-dependent manners. The cellular uptake of [C60(C(COOH) 2)2]n nanoparticles was found to be clathrin-mediated but not caveolae-mediated endocytosis. The endocytosis mechanism and the subcellular target location provide key information for the better understanding and predicting of the biomedical function of fullerene nanoparticles inside cells.

Original languageEnglish (US)
Article number145102
JournalNanotechnology
Volume19
Issue number14
DOIs
StatePublished - Apr 9 2008

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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