The topology of the mitochondrial 11β-hydroxylase system in bovine adrenal cortex

J. Rydström; J. Å Gustafsson; M. Ingelman-Sundberg; J. Montelius; L. Ernster

doi:10.1016/0006-291X(76)90846-9

The topology of the mitochondrial 11β-hydroxylase system in bovine adrenal cortex

J. Rydström, J. Å Gustafsson, M. Ingelman-Sundberg, J. Montelius, L. Ernster

Houston Methodist

Research output: Contribution to journal › Article › peer-review

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Abstract

Binding of deoxycorticosterone to cytochrome P-450 of the 11β-hydroxylase system in adrenal cortex mitochondria was inhibited by the nonpenetrating protein reagent diazobenzenesulfonate in damaged but not in intact mitochondria. The slowly penetrating hydrophilic substrate deoxycorticosterone 21-sulfate showed a slow binding to cytochrome P-450 as compared to the hydrophobic nonesterified steroid. In contrast, the esterified and nonesterified steroids bound equally fast in sonicated, aged or lysolecithin-treated mitochondria. These data imply that the steroid substrates must penetrate the inner mitochondrial membrane to interact with the 11β-hydroxylase system.

Original language	English (US)
Pages (from-to)	555-561
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	73
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(76)90846-9
State	Published - Dec 6 1976

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "Binding of deoxycorticosterone to cytochrome P-450 of the 11β-hydroxylase system in adrenal cortex mitochondria was inhibited by the nonpenetrating protein reagent diazobenzenesulfonate in damaged but not in intact mitochondria. The slowly penetrating hydrophilic substrate deoxycorticosterone 21-sulfate showed a slow binding to cytochrome P-450 as compared to the hydrophobic nonesterified steroid. In contrast, the esterified and nonesterified steroids bound equally fast in sonicated, aged or lysolecithin-treated mitochondria. These data imply that the steroid substrates must penetrate the inner mitochondrial membrane to interact with the 11β-hydroxylase system.",

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AU - Gustafsson, J. Å

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AU - Montelius, J.

AU - Ernster, L.

PY - 1976/12/6

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AB - Binding of deoxycorticosterone to cytochrome P-450 of the 11β-hydroxylase system in adrenal cortex mitochondria was inhibited by the nonpenetrating protein reagent diazobenzenesulfonate in damaged but not in intact mitochondria. The slowly penetrating hydrophilic substrate deoxycorticosterone 21-sulfate showed a slow binding to cytochrome P-450 as compared to the hydrophobic nonesterified steroid. In contrast, the esterified and nonesterified steroids bound equally fast in sonicated, aged or lysolecithin-treated mitochondria. These data imply that the steroid substrates must penetrate the inner mitochondrial membrane to interact with the 11β-hydroxylase system.

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The topology of the mitochondrial 11β-hydroxylase system in bovine adrenal cortex

Abstract

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