The topology of the mitochondrial 11β-hydroxylase system in bovine adrenal cortex

J. Rydström, J. Å Gustafsson, M. Ingelman-Sundberg, J. Montelius, L. Ernster

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Binding of deoxycorticosterone to cytochrome P-450 of the 11β-hydroxylase system in adrenal cortex mitochondria was inhibited by the nonpenetrating protein reagent diazobenzenesulfonate in damaged but not in intact mitochondria. The slowly penetrating hydrophilic substrate deoxycorticosterone 21-sulfate showed a slow binding to cytochrome P-450 as compared to the hydrophobic nonesterified steroid. In contrast, the esterified and nonesterified steroids bound equally fast in sonicated, aged or lysolecithin-treated mitochondria. These data imply that the steroid substrates must penetrate the inner mitochondrial membrane to interact with the 11β-hydroxylase system.

Original languageEnglish (US)
Pages (from-to)555-561
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume73
Issue number3
DOIs
StatePublished - Dec 6 1976

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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