TY - JOUR
T1 - The temperature-sensitive role of Cryptococcus neoformans ROM2 in cell morphogenesis
AU - Fuchs, Beth Burgwyn
AU - Tang, Robin J.
AU - Mylonakis, Eleftherios
PY - 2007/4/11
Y1 - 2007/4/11
N2 - ROM2 is associated with Cryptococcus neoformants virulence. We examined additional roles of ROM2 in C. neoformans and found that ROM2 plays a role in several cell functions specifically at high temperature conditions. Morphologically rom2 mutant cells demonstrated a "tear"-like shape and clustered together. A sub-population of cells had a hyperelongated phenotype at restrictive growth conditions. Altered morphology was associated with defects in actin that was concentrated at the cell periphery and with abnormalities in microtubule organization. Interestingly, the ROM2 associated defects in cell morphology, location of nuclei, and actin and microtubule organization were not observed in cells grown at temperatures below 37°C. These results indicate that in C. neoformans, ROM2 is important at restrictive temperature conditions and is involved in several cell maintenance functions.
AB - ROM2 is associated with Cryptococcus neoformants virulence. We examined additional roles of ROM2 in C. neoformans and found that ROM2 plays a role in several cell functions specifically at high temperature conditions. Morphologically rom2 mutant cells demonstrated a "tear"-like shape and clustered together. A sub-population of cells had a hyperelongated phenotype at restrictive growth conditions. Altered morphology was associated with defects in actin that was concentrated at the cell periphery and with abnormalities in microtubule organization. Interestingly, the ROM2 associated defects in cell morphology, location of nuclei, and actin and microtubule organization were not observed in cells grown at temperatures below 37°C. These results indicate that in C. neoformans, ROM2 is important at restrictive temperature conditions and is involved in several cell maintenance functions.
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U2 - 10.1371/journal.pone.0000368
DO - 10.1371/journal.pone.0000368
M3 - Article
C2 - 17426816
AN - SCOPUS:55449118602
VL - 2
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 4
M1 - e368
ER -