The specific binding of peptide ligands to cardiomyocytes derived from mouse embryonic stem cells

Zhuokun Li, Jiusong Fan, Wenxiu Zhao, Lei Jin, Lan Ma

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Purification of pluripotent stem cell (PSC)-derived cardiomyocytes is critical for the application of cardiomyocytes both in clinical and basic research. Finding a specific cell marker is a promising method for purifying induced cells. The present study employed phage display technology to search for particular cell markers that could bind specifically to PSC-derived cardiomyocytes. After three rounds of biopanning, several peptides were obtained. The ELISA results show the no. 3 sequence peptide (QPFTTSLTPPAR), and other four sequences having a consensus motif [SS(Q)PPQ(S)], no. 9, 11, 14, and 10, have relatively high affinity and specificity to cardiomyocytes. Immunofluorescence confirmed that the selected peptides could bind specifically to the PSC-derived cardiomyocytes. Competition tests with chemically synthesized peptides revealed the binding ability was caused by the peptide itself. Western blot analysis proved the phages were both bound to two 17kDa cardiomyocyte membrane proteins and the no. 9 sequence showed a 55kDa protein that was not observed in the no. 3 sequence. These results suggest that the selected peptides specifically target receptors on PSC-derived cardiomyocyte membranes. The results will pave the way for further studies of cell surface markers and their applications, such as labeling, purification, and as vehicles for drug delivery.

Original languageEnglish (US)
Pages (from-to)771-782
Number of pages12
JournalJournal of Peptide Science
Volume17
Issue number12
DOIs
StatePublished - Dec 2011

Keywords

  • Cardiomyocytes
  • Embryoid body
  • In vitro differentiation
  • Mouse embryonic stem cell
  • Peptide
  • Phage display library

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Medicine
  • Molecular Biology
  • Biochemistry
  • Pharmacology
  • Drug Discovery
  • Organic Chemistry

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