The scavenging of reactive oxygen species and the potential for cell protection by functionalized fullerene materials

Jun Jie Yin; Fang Lao; Peter P. Fu; Wayne G. Wamer; Yuliang Zhao; Paul C. Wang; Yang Qiu; Baoyun Sun; Gengmei Xing; Jinquan Dong; Xing Jie Liang; Chunying Chen

doi:10.1016/j.biomaterials.2008.09.061

The scavenging of reactive oxygen species and the potential for cell protection by functionalized fullerene materials

Jun Jie Yin, Fang Lao, Peter P. Fu, Wayne G. Wamer, Yuliang Zhao, Paul C. Wang, Yang Qiu, Baoyun Sun, Gengmei Xing, Jinquan Dong, Xing Jie Liang, Chunying Chen

Research output: Contribution to journal › Article › peer-review

420 Scopus citations

Abstract

We demonstrated that three different types of water-soluble fullerenes materials can intercept all of the major physiologically relevant ROS. C₆₀(C(COOH)₂)₂, C₆₀(OH)₂₂, and Gd@C₈₂(OH)₂₂ can protect cells against H₂O₂-induced oxidative damage, stabilize the mitochondrial membrane potential and reduce intracellular ROS production with the following relative potencies: Gd@C₈₂(OH)₂₂ ≥ C₆₀(OH)₂₂ > C₆₀(C(COOH)₂)₂. Consistent with their cytoprotective abilities, these derivatives can scavenge the stable 2,2-diphenyl-1-picryhydrazyl radical (DPPH), and the reactive oxygen species (ROS) superoxide radical anion (O₂ ^{{radical dot}-}), singlet oxygen, and hydroxyl radical (HO^{{radical dot}}), and can also efficiently inhibit lipid peroxidation in vitro. The observed differences in free radical-scavenging capabilities support the hypothesis that both chemical properties, such as surface chemistry induced differences in electron affinity, and physical properties, such as degree of aggregation, influence the biological and biomedical activities of functionalized fullerenes. This represents the first report that different types of fullerene derivatives can scavenge all physiologically relevant ROS. The role of oxidative stress and damage in the etiology and progression of many diseases suggests that these fullerene derivatives may be valuable in vivo cytoprotective and therapeutic agents.

Original language	English (US)
Pages (from-to)	611-621
Number of pages	11
Journal	Biomaterials
Volume	30
Issue number	4
DOIs	https://doi.org/10.1016/j.biomaterials.2008.09.061
State	Published - Feb 2009

Keywords

Carboxyfullerene
Cytoprotection
Fullerenol
Gadolinium endohedral metallofullerenol
Reactive oxygen species
Scavenging activity

ASJC Scopus subject areas

Biomaterials
Bioengineering
Ceramics and Composites
Mechanics of Materials
Biophysics

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10.1016/j.biomaterials.2008.09.061

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@article{f9c53c203c64465a81e7e830eae5c5a7,

title = "The scavenging of reactive oxygen species and the potential for cell protection by functionalized fullerene materials",

abstract = "We demonstrated that three different types of water-soluble fullerenes materials can intercept all of the major physiologically relevant ROS. C60(C(COOH)2)2, C60(OH)22, and Gd@C82(OH)22 can protect cells against H2O2-induced oxidative damage, stabilize the mitochondrial membrane potential and reduce intracellular ROS production with the following relative potencies: Gd@C82(OH)22 ≥ C60(OH)22 > C60(C(COOH)2)2. Consistent with their cytoprotective abilities, these derivatives can scavenge the stable 2,2-diphenyl-1-picryhydrazyl radical (DPPH), and the reactive oxygen species (ROS) superoxide radical anion (O2 {radical dot}-), singlet oxygen, and hydroxyl radical (HO{radical dot}), and can also efficiently inhibit lipid peroxidation in vitro. The observed differences in free radical-scavenging capabilities support the hypothesis that both chemical properties, such as surface chemistry induced differences in electron affinity, and physical properties, such as degree of aggregation, influence the biological and biomedical activities of functionalized fullerenes. This represents the first report that different types of fullerene derivatives can scavenge all physiologically relevant ROS. The role of oxidative stress and damage in the etiology and progression of many diseases suggests that these fullerene derivatives may be valuable in vivo cytoprotective and therapeutic agents.",

keywords = "Carboxyfullerene, Cytoprotection, Fullerenol, Gadolinium endohedral metallofullerenol, Reactive oxygen species, Scavenging activity",

author = "Yin, {Jun Jie} and Fang Lao and Fu, {Peter P.} and Wamer, {Wayne G.} and Yuliang Zhao and Wang, {Paul C.} and Yang Qiu and Baoyun Sun and Gengmei Xing and Jinquan Dong and Liang, {Xing Jie} and Chunying Chen",

note = "Funding Information: This work is financially supported by the Chinese Academy of Sciences (CAS) “Hundred Talents Program” (07165111ZX), the National Basic Research Program of China (2006CB705603, 2007CB935604), the Natural Science Foundation of China (NSFC) (10525524, 20751001, and 10490180), and the CAS Knowledge Innovation Program. This work was also supported in part by NIH/NCRR/RCMI 2G12RR003048, NIH 5U 54CA091431, and USAMRMC W81XWH-05-1-0291 grants. ",

year = "2009",

month = feb,

doi = "10.1016/j.biomaterials.2008.09.061",

language = "English (US)",

volume = "30",

pages = "611--621",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier BV",

number = "4",

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TY - JOUR

T1 - The scavenging of reactive oxygen species and the potential for cell protection by functionalized fullerene materials

AU - Yin, Jun Jie

AU - Lao, Fang

AU - Fu, Peter P.

AU - Wamer, Wayne G.

AU - Zhao, Yuliang

AU - Wang, Paul C.

AU - Qiu, Yang

AU - Sun, Baoyun

AU - Xing, Gengmei

AU - Dong, Jinquan

AU - Liang, Xing Jie

AU - Chen, Chunying

N1 - Funding Information: This work is financially supported by the Chinese Academy of Sciences (CAS) “Hundred Talents Program” (07165111ZX), the National Basic Research Program of China (2006CB705603, 2007CB935604), the Natural Science Foundation of China (NSFC) (10525524, 20751001, and 10490180), and the CAS Knowledge Innovation Program. This work was also supported in part by NIH/NCRR/RCMI 2G12RR003048, NIH 5U 54CA091431, and USAMRMC W81XWH-05-1-0291 grants.

PY - 2009/2

Y1 - 2009/2

N2 - We demonstrated that three different types of water-soluble fullerenes materials can intercept all of the major physiologically relevant ROS. C60(C(COOH)2)2, C60(OH)22, and Gd@C82(OH)22 can protect cells against H2O2-induced oxidative damage, stabilize the mitochondrial membrane potential and reduce intracellular ROS production with the following relative potencies: Gd@C82(OH)22 ≥ C60(OH)22 > C60(C(COOH)2)2. Consistent with their cytoprotective abilities, these derivatives can scavenge the stable 2,2-diphenyl-1-picryhydrazyl radical (DPPH), and the reactive oxygen species (ROS) superoxide radical anion (O2 {radical dot}-), singlet oxygen, and hydroxyl radical (HO{radical dot}), and can also efficiently inhibit lipid peroxidation in vitro. The observed differences in free radical-scavenging capabilities support the hypothesis that both chemical properties, such as surface chemistry induced differences in electron affinity, and physical properties, such as degree of aggregation, influence the biological and biomedical activities of functionalized fullerenes. This represents the first report that different types of fullerene derivatives can scavenge all physiologically relevant ROS. The role of oxidative stress and damage in the etiology and progression of many diseases suggests that these fullerene derivatives may be valuable in vivo cytoprotective and therapeutic agents.

AB - We demonstrated that three different types of water-soluble fullerenes materials can intercept all of the major physiologically relevant ROS. C60(C(COOH)2)2, C60(OH)22, and Gd@C82(OH)22 can protect cells against H2O2-induced oxidative damage, stabilize the mitochondrial membrane potential and reduce intracellular ROS production with the following relative potencies: Gd@C82(OH)22 ≥ C60(OH)22 > C60(C(COOH)2)2. Consistent with their cytoprotective abilities, these derivatives can scavenge the stable 2,2-diphenyl-1-picryhydrazyl radical (DPPH), and the reactive oxygen species (ROS) superoxide radical anion (O2 {radical dot}-), singlet oxygen, and hydroxyl radical (HO{radical dot}), and can also efficiently inhibit lipid peroxidation in vitro. The observed differences in free radical-scavenging capabilities support the hypothesis that both chemical properties, such as surface chemistry induced differences in electron affinity, and physical properties, such as degree of aggregation, influence the biological and biomedical activities of functionalized fullerenes. This represents the first report that different types of fullerene derivatives can scavenge all physiologically relevant ROS. The role of oxidative stress and damage in the etiology and progression of many diseases suggests that these fullerene derivatives may be valuable in vivo cytoprotective and therapeutic agents.

KW - Carboxyfullerene

KW - Cytoprotection

KW - Fullerenol

KW - Gadolinium endohedral metallofullerenol

KW - Reactive oxygen species

KW - Scavenging activity

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U2 - 10.1016/j.biomaterials.2008.09.061

DO - 10.1016/j.biomaterials.2008.09.061

M3 - Article

C2 - 18986699

AN - SCOPUS:56449101145

SN - 0142-9612

VL - 30

SP - 611

EP - 621

JO - Biomaterials

JF - Biomaterials

IS - 4

ER -

The scavenging of reactive oxygen species and the potential for cell protection by functionalized fullerene materials

Abstract

Keywords

ASJC Scopus subject areas

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