The scavenging of reactive oxygen species and the potential for cell protection by fullerenols with different isoelectric points

Fullerenols were known as the major water-soluble derivatives of fullerene that possess particular significance biological effects such as anticancer active and free radical scavengers. We have reported a novel method for purifying nanoparticles of fullerenols, which based on different isoelectric points (pls). Consistent with their cytoprotective abilities, these samples can scavenge the stable 2,2-diphenyl-1-picryhydrazyl (DPPH) radical in vitro with the following relative potencies: pl 7.0 > pl 5.29 > pl 4.40 > pl 2.81. The observed differences in free radical-scavenging capabilities support the hypothesis that physicochemical properties, such as surface chemistry induced differences in electron affinity, and degree of aggregation, influence the biological and biomedical activities of fullerenols with different pls. These purified fullerenols with pl 2.81, pl 4.40, pl 5.29 and pl 7.0 were performed to determine the effect of purified fullerenols on cytotoxicity and DNA stability in Raw 264.7 cells. There no significantly different by statistical product and service solutions (SPSS) analysis. But only the sample of fullerenols with pl 2.81 caused obvious DNA damage to RAW264.7 cells in the continuous buffer system.