The Role of the CLIC/GEEC Endocytic Pathway for Mechanophysical Transfection of DNA

Sean Weaver; Emran O Lallow; Kelly Kyker-Snowman; Nandita C Jhumur; Melissa Gulley; Christine C Roberts; David I Shreiber; Hao Lin; Joel N Maslow

doi:10.1002/jgm.70059

The Role of the CLIC/GEEC Endocytic Pathway for Mechanophysical Transfection of DNA

Sean Weaver, Emran O Lallow, Kelly Kyker-Snowman, Nandita C Jhumur, Melissa Gulley, Christine C Roberts, David I Shreiber, Hao Lin, Joel N Maslow

Research output: Contribution to journal › Review article › peer-review

Abstract

The clathrin-independent carriers/glycosylphosphatidylinositol-attached protein-enriched endosomal compartments (CLIC/GEEC) pathway is a clarithin- and dynamin-independent endocytic pathway. CLIC/GEEC-mediated endocytosis is a large-capacity, rapid-acting process that is utilized by cells for bulk uptake of nutrients and membrane-anchored cargo and for homeostatic maintenance of membrane tension. Here, we present a brief overview of this pathway, including its known molecular mechanisms, inhibitors, activity in different cell types, and its potential relevance in mechanophysical DNA transfection.

Original language	English (US)
Article number	e70059
Journal	Journal of Gene Medicine
Volume	27
Issue number	12
DOIs	https://doi.org/10.1002/jgm.70059
State	Published - Dec 2025

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Genetics
Drug Discovery
Genetics(clinical)

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title = "The Role of the CLIC/GEEC Endocytic Pathway for Mechanophysical Transfection of DNA",

abstract = "The clathrin-independent carriers/glycosylphosphatidylinositol-attached protein-enriched endosomal compartments (CLIC/GEEC) pathway is a clarithin- and dynamin-independent endocytic pathway. CLIC/GEEC-mediated endocytosis is a large-capacity, rapid-acting process that is utilized by cells for bulk uptake of nutrients and membrane-anchored cargo and for homeostatic maintenance of membrane tension. Here, we present a brief overview of this pathway, including its known molecular mechanisms, inhibitors, activity in different cell types, and its potential relevance in mechanophysical DNA transfection.",

author = "Sean Weaver and Emran O Lallow and Kelly Kyker-Snowman and Nandita C Jhumur and Melissa Gulley and Christine C Roberts and David I Shreiber and Hao Lin and Joel N Maslow",

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AU - Weaver, Sean

AU - Lallow, Emran O

AU - Kyker-Snowman, Kelly

AU - Jhumur, Nandita C

AU - Gulley, Melissa

AU - Roberts, Christine C

AU - Shreiber, David I

AU - Lin, Hao

AU - Maslow, Joel N

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N2 - The clathrin-independent carriers/glycosylphosphatidylinositol-attached protein-enriched endosomal compartments (CLIC/GEEC) pathway is a clarithin- and dynamin-independent endocytic pathway. CLIC/GEEC-mediated endocytosis is a large-capacity, rapid-acting process that is utilized by cells for bulk uptake of nutrients and membrane-anchored cargo and for homeostatic maintenance of membrane tension. Here, we present a brief overview of this pathway, including its known molecular mechanisms, inhibitors, activity in different cell types, and its potential relevance in mechanophysical DNA transfection.

AB - The clathrin-independent carriers/glycosylphosphatidylinositol-attached protein-enriched endosomal compartments (CLIC/GEEC) pathway is a clarithin- and dynamin-independent endocytic pathway. CLIC/GEEC-mediated endocytosis is a large-capacity, rapid-acting process that is utilized by cells for bulk uptake of nutrients and membrane-anchored cargo and for homeostatic maintenance of membrane tension. Here, we present a brief overview of this pathway, including its known molecular mechanisms, inhibitors, activity in different cell types, and its potential relevance in mechanophysical DNA transfection.

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M3 - Review article

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VL - 27

JO - Journal of Gene Medicine

JF - Journal of Gene Medicine

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The Role of the CLIC/GEEC Endocytic Pathway for Mechanophysical Transfection of DNA

Abstract

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