The Role of Escherichia coli dnaG Function in Coliphage M 13 DNA Synthesis

Santanu DASGUPTA; Saukar MITRA

doi:10.1111/j.1432-1033.1976.tb10630.x

The Role of Escherichia coli dnaG Function in Coliphage M 13 DNA Synthesis

Santanu DASGUPTA, Saukar MITRA

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2 Scopus citations

Abstract

Examination of the role of Escherichia coli dnaG function in different stages of M13 phage DNA synthesis by ultracentrifugal analysis of intracellular phage DNA in a thermosensitive dnaG mutant shows that: the formation of parental double strand replicative form DNA (rfDNA) from the infecting virus is independent of dnaG function; the synthesis of progeny rfDNA requires dnaG product; after a pool of rfDNA is made up, dnaG function is not required for the progeny single strand DNA (ssDNA) synthesis. The ssDNAs produced under nonpermissive condition are mostly circular and biologically functional.

Original language	English (US)
Pages (from-to)	47-51
Number of pages	5
Journal	European Journal of Biochemistry
Volume	67
Issue number	1
DOIs	https://doi.org/10.1111/j.1432-1033.1976.tb10630.x
State	Published - Aug 1976

ASJC Scopus subject areas

Biochemistry

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abstract = "Examination of the role of Escherichia coli dnaG function in different stages of M13 phage DNA synthesis by ultracentrifugal analysis of intracellular phage DNA in a thermosensitive dnaG mutant shows that: the formation of parental double strand replicative form DNA (rfDNA) from the infecting virus is independent of dnaG function; the synthesis of progeny rfDNA requires dnaG product; after a pool of rfDNA is made up, dnaG function is not required for the progeny single strand DNA (ssDNA) synthesis. The ssDNAs produced under nonpermissive condition are mostly circular and biologically functional.",

author = "Santanu DASGUPTA and Saukar MITRA",

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AU - MITRA, Saukar

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AB - Examination of the role of Escherichia coli dnaG function in different stages of M13 phage DNA synthesis by ultracentrifugal analysis of intracellular phage DNA in a thermosensitive dnaG mutant shows that: the formation of parental double strand replicative form DNA (rfDNA) from the infecting virus is independent of dnaG function; the synthesis of progeny rfDNA requires dnaG product; after a pool of rfDNA is made up, dnaG function is not required for the progeny single strand DNA (ssDNA) synthesis. The ssDNAs produced under nonpermissive condition are mostly circular and biologically functional.

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