The regulation of NK cells cytotoxicity by SNAP-23

Fang Zheng, Qun Wu, Dong Fang Liu, Ping Xiong, Fei Li Gong

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the regulation of NK cells cytotoxicity by SNAP-23. Methods: The expression of SNAP-23 was silenced by specific shRNA plasmid transfection using nucleofector. LDH release assay and β-hexosaminidase release assay were respectively used to detect the change of NK92 cytotoxicity and exocytosis after suppression of SNAP-23. Results: Specific shRNA could suppress the expression of SNAP-23 in NK92 cells. After that, the cytotoxicity and exocytosis of NK92 cells decreased. Conclusion: SNAP-23 can regulate the cytotoxicity through modulating exocytosis of NK cells.

Original languageEnglish (US)
Pages (from-to)97-100
Number of pages4
JournalChinese Journal of Microbiology and Immunology
Volume26
Issue number2
StatePublished - Feb 28 2006

Keywords

  • Cytotoxicity
  • Exocytosis
  • NK cell
  • SNAP-23
  • shRNA

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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