TY - JOUR
T1 - The reduced bactericidal function of complement C5-deficient murine macrophages is associated with defects in the synthesis and delivery of reactive oxygen radicals to mycobacterial phagosomes
AU - Daniel, D. Sundarsingh
AU - Dai, Guixiang
AU - Singh, Christopher R.
AU - Lindsey, Devin R.
AU - Smith, Amanda K.
AU - Dhandayuthapani, Subramanian
AU - Hunter, Robert L.
AU - Jagannath, Chinnaswamy
PY - 2006/10/1
Y1 - 2006/10/1
N2 - Complement C5-deficient (C5-/-) macrophages derived from B.10 congenic mice ware found to be defective to killing intracellular Mycobacterium tuberculosis (MTB). They were bacteriostatic after activation with IFN-γ alone but bactericidal in the combined presence of IFN-γ and C5-derived C5a anaphylatoxin that was deficient among these macrophages. Reduced killing correlated with a decreased production of reactive oxygen species (ROS) in the C5-/- macrophages measured using fluorescent probes. Furthermore, a lack of colocalizatlon of p47phox protein of the NADPH oxidase (phox) complex with GFP-espressing MTB (gfpMTB) indicated a defective assembly of the phox complex on phagosomes. Reconstitution with C5a, a known ROS activator, enhanced the assembly of phox complex on the phagosomes as well as the production of ROS that inhibited the growth of MTB. Protein kinase C (PKC) isoforms are involved in the phosphorylation and translocation of p47 phox onto bacterial phagosomes. Western blot analysis demonstrated a defective phosphorylation of PKC (α, β, δ) and PKC-ζ in the cytosol of C5-/- macrophages compared with C5 intact (C5 +/+) macrophages. Furthermore, in site fluorescent labeling of phagosomes indicated that PKC-β and PKC-ζ were the isoforms that are not phosphorylated in C5-/- macrophages. Because Fc receptor-mediated phox assembly was normal in both C5-/- and C5+/+ macrophages, the defect in phox assembly around MTB phagosomes was specific to C5 deficiency. Reduced bactericidal function of C5-/- macrophages thus appears to be due to a defective assembly and production of ROS that prevents effective killing of intracellular MTB.
AB - Complement C5-deficient (C5-/-) macrophages derived from B.10 congenic mice ware found to be defective to killing intracellular Mycobacterium tuberculosis (MTB). They were bacteriostatic after activation with IFN-γ alone but bactericidal in the combined presence of IFN-γ and C5-derived C5a anaphylatoxin that was deficient among these macrophages. Reduced killing correlated with a decreased production of reactive oxygen species (ROS) in the C5-/- macrophages measured using fluorescent probes. Furthermore, a lack of colocalizatlon of p47phox protein of the NADPH oxidase (phox) complex with GFP-espressing MTB (gfpMTB) indicated a defective assembly of the phox complex on phagosomes. Reconstitution with C5a, a known ROS activator, enhanced the assembly of phox complex on the phagosomes as well as the production of ROS that inhibited the growth of MTB. Protein kinase C (PKC) isoforms are involved in the phosphorylation and translocation of p47 phox onto bacterial phagosomes. Western blot analysis demonstrated a defective phosphorylation of PKC (α, β, δ) and PKC-ζ in the cytosol of C5-/- macrophages compared with C5 intact (C5 +/+) macrophages. Furthermore, in site fluorescent labeling of phagosomes indicated that PKC-β and PKC-ζ were the isoforms that are not phosphorylated in C5-/- macrophages. Because Fc receptor-mediated phox assembly was normal in both C5-/- and C5+/+ macrophages, the defect in phox assembly around MTB phagosomes was specific to C5 deficiency. Reduced bactericidal function of C5-/- macrophages thus appears to be due to a defective assembly and production of ROS that prevents effective killing of intracellular MTB.
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U2 - 10.4049/jimmunol.177.7.4688
DO - 10.4049/jimmunol.177.7.4688
M3 - Article
C2 - 16982908
AN - SCOPUS:33749142975
VL - 177
SP - 4688
EP - 4698
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 7
ER -