The promise and challenge of high-throughput sequencing of the antibody repertoire

George Georgiou, Gregory C. Ippolito, John Beausang, Christian E. Busse, Hedda Wardemann, Stephen R. Quake

Research output: Contribution to journalReview articlepeer-review

395 Scopus citations

Abstract

Efforts to determine the antibody repertoire encoded by B cells in the blood or lymphoid organs using high-throughput DNA sequencing technologies have been advancing at an extremely rapid pace and are transforming our understanding of humoral immune responses. Information gained from high-throughput DNA sequencing of immunoglobulin genes (Ig-seq) can be applied to detect B-cell malignancies with high sensitivity, to discover antibodies specific for antigens of interest, to guide vaccine development and to understand autoimmunity. Rapid progress in the development of experimental protocols and informatics analysis tools is helping to reduce sequencing artifacts, to achieve more precise quantification of clonal diversity and to extract the most pertinent biological information. That said, broader application of Ig-seq, especially in clinical settings, will require the development of a standardized experimental design framework that will enable the sharing and meta-analysis of sequencing data generated by different laboratories.

Original languageEnglish (US)
Pages (from-to)158-168
Number of pages11
JournalNature Biotechnology
Volume32
Issue number2
DOIs
StatePublished - Feb 2014

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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