TY - JOUR
T1 - The preterm placental microbiome varies in association with excess maternal gestational weight gain
AU - Antony, Kathleen M.
AU - Ma, Jun
AU - Mitchell, Kristen B.
AU - Racusin, Diana A.
AU - Versalovic, James
AU - Aagaard, Kjersti
N1 - Funding Information:
Supported by National Institutes of Health Director’s New Innovator Award number DP2 DP21DP2OD001500, Burroughs Welcome Fund PTB Initiative grant number 1008819.01, and National Institute of Nursing Research Grant number R01NR014792 (all to K.A.) and the Human Microbiome Project funded through the National Institutes of Health Director’s Common Fund. All sequencing and adaptation of protocols for sequencing low-biomass samples were performed by the Baylor College of Medicine Human Genome Sequencing Center, which is funded by direct support from the National Human Genome Research Institute , National Institutes of Health , under grant number U54HG004973 .
Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Objective Although a higher maternal body mass index is associated with preterm birth, it is unclear whether excess gestational weight gain (GWG) or obesity drives increased risk. We and others have shown that the placenta harbors microbiota, which is significantly different among preterm births. Our aim in this study was to investigate whether the preterm placental microbiome varies by virtue of obesity or alternately by excess GWG. Study Design Placentas (n = 320) were collected from term and preterm pregnancies. Genomic DNA was extracted and subjected to metagenomic sequencing. Data were analyzed by clinical covariates that included the 2009 Institute of Medicine's GWG guideline and obesity. Results Analysis of 16S recombinant RNA-based metagenomics revealed no clustering of the microbiome by virtue of obesity (P =.161). Among women who spontaneously delivered preterm, there was again no clustering by obesity (P =.480), but there was significant clustering by excess GWG (P =.022). Moreover, among preterm births, detailed analysis identified microbial genera (family and genus level) and bacterial metabolic gene pathways that varied among pregnancies with excess GWG. Notably, excess GWG was associated with decreased microbial folate biosynthesis pathways and decreased butanoate metabolism (linear discriminate analysis, >3.0-fold). Conclusion Although there were no significant alterations in the microbiome by virtue of obesity per se, excess GWG was associated with an altered microbiome and its metabolic profile among those women who experienced a preterm birth.
AB - Objective Although a higher maternal body mass index is associated with preterm birth, it is unclear whether excess gestational weight gain (GWG) or obesity drives increased risk. We and others have shown that the placenta harbors microbiota, which is significantly different among preterm births. Our aim in this study was to investigate whether the preterm placental microbiome varies by virtue of obesity or alternately by excess GWG. Study Design Placentas (n = 320) were collected from term and preterm pregnancies. Genomic DNA was extracted and subjected to metagenomic sequencing. Data were analyzed by clinical covariates that included the 2009 Institute of Medicine's GWG guideline and obesity. Results Analysis of 16S recombinant RNA-based metagenomics revealed no clustering of the microbiome by virtue of obesity (P =.161). Among women who spontaneously delivered preterm, there was again no clustering by obesity (P =.480), but there was significant clustering by excess GWG (P =.022). Moreover, among preterm births, detailed analysis identified microbial genera (family and genus level) and bacterial metabolic gene pathways that varied among pregnancies with excess GWG. Notably, excess GWG was associated with decreased microbial folate biosynthesis pathways and decreased butanoate metabolism (linear discriminate analysis, >3.0-fold). Conclusion Although there were no significant alterations in the microbiome by virtue of obesity per se, excess GWG was associated with an altered microbiome and its metabolic profile among those women who experienced a preterm birth.
KW - Excess gestational
KW - obesity metagenomics microbiome
KW - preterm birth
KW - weight gain maternal
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U2 - 10.1016/j.ajog.2014.12.041
DO - 10.1016/j.ajog.2014.12.041
M3 - Article
C2 - 25557210
AN - SCOPUS:84928825527
SN - 0002-9378
VL - 212
SP - 653.e1-653.e16
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
IS - 5
ER -