The novel peptide apelin regulates intrarenal artery tone in diabetic mice

Jiu Chang Zhong, Yu Huang, Lai Ming Yung, Chi Wai Lau, Fung Ping Leung, Wing Tak Wong, Shu Guang Lin, Xi Yong Yu

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Apelin, a newly identified angiotensin (Ang) II homologue, has been implicated in diabetes. We previously reported that apelin exerts an opposing influence on the Ang II signaling. Our aim was to further implore whether apelin could regulate intrarenal artery tone in response to Ang II and Ang IV in diabetes. A Multi Myograph system was used to determine the isometric renal artery tone in diabetic db/db and control db/m + mice. The phosphorylation, and protein levels of endothelial nitric oxide (NO) synthase (eNOS), and apelin receptor APJ were analyzed by Western blotting. Diminished expression of APJ protein and enhanced contractile responses to Ang II and Ang IV were exhibited in renal arteries from db/db mice. Apelin supplement reversed the abnormal renal vascular responsiveness to Ang II and acetylcholine, but not to Ang IV in db/db mice. Finally, in db/db mice, significant increases in phosphorylation of eNOS on serine 1177 and in NO generation were found in renal arteries pretreated with apelin. Our findings provide novel evidence for the regulatory roles of renal apelin system in vascular functions in diabetes. Apelin treatment may regulate the balance between Ang II and NO and thereby exert beneficial effects on the diabetic vascular pathophysiology.

Original languageEnglish (US)
Pages (from-to)109-114
Number of pages6
JournalRegulatory Peptides
Volume144
Issue number1-3
DOIs
StatePublished - Dec 1 2007

Keywords

  • APJ receptor
  • Angiotensin II
  • Apelin
  • Artery tone
  • Diabetes
  • Nitric oxide

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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