The non-activated glucocorticoid receptor: Structure and activation

Jan Åke Gustafsson, Ann Charlotte Wikström, Marc Denis

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Glucocorticoid hormone receptors are present in the soluble fraction of target cell homogenates as large entities (Mr ≈ 300,000) that are unable to interact with DNA. These large complexes contain an Mr ≈ 94,000 steroid- and DNA-binding polypeptide, in association with an Mr ≈ 90,000 non-ligand-binding entity, which has been identified as a heat shock protein, hsp90. This protein has been purified to near homogeneity as a component of the non-activated receptor complex. Characterization of the purified protein revealed its presence as a dimer in the large receptor form. Dissociation of the receptor-hsp90 complex can be induced by heat treatment only when ligand is bound to the receptor, as demonstrated by specific DNA-binding assay and sucrose gradient ultracentrifugation. hsp90 represents ca 1 % of total proteins in rat liver cytosol, and milligram amounts were purified using a combination of high performance ion exchange and gel permeation chromatography. Monospecific antibodies were raised in rabbits. They were found to precipitate the intact non-activated glucocorticoid receptor, as well as the Mr ≈ 27,000 steroid-binding fragment of the receptor generated by trypsin treatment, indicating that hsp90 interacts with the steroid-binding domain of the glucocorticoid receptor. Finally, translation of glucocorticoid receptor mRNA in reticulocyte lysate yields a protein which also interacts with hsp90 and binds to DNA only after ligand-binding and heat treatment. Thus, the glucocorticoid receptor is synthesized in a non-activated form also in vitro.

Original languageEnglish (US)
Pages (from-to)53-62
Number of pages10
JournalJournal of Steroid Biochemistry
Volume34
Issue number1-6
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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