The modulation of endothelial cell morphology, function, and survival using anisotropic nanofibrillar collagen scaffolds

Ngan F. Huang, Janet Okogbaa, Jerry C. Lee, Arshi Jha, Tatiana S. Zaitseva, Michael V. Paukshto, John S. Sun, Niraj Punjya, Gerald G. Fuller, John P. Cooke

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Endothelial cells (ECs) are aligned longitudinally under laminar flow, whereas they are polygonal and poorly aligned in regions of disturbed flow. The unaligned ECs in disturbed flow fields manifest altered function and reduced survival that promote lesion formation. We demonstrate that the alignment of the ECs may directly influence their biology, independent of fluid flow. We developed aligned nanofibrillar collagen scaffolds that mimic the structure of collagen bundles in blood vessels, and examined the effects of these materials on EC alignment, function, and in vivo survival. ECs cultured on 30-nm diameter aligned fibrils re-organized their F-actin along the nanofibril direction, and were 50% less adhesive for monocytes than the ECs grown on randomly oriented fibrils. After EC transplantation into both subcutaneous tissue and the ischemic hindlimb, EC viability was enhanced when ECs were cultured and implanted on aligned nanofibrillar scaffolds, in contrast to non-patterned scaffolds. ECs derived from human induced pluripotent stem cells and cultured on aligned scaffolds also persisted for over 28 days, as assessed by bioluminescence imaging, when implanted in ischemic tissue. By contrast, ECs implanted on scaffolds without nanopatterning generated no detectable bioluminescent signal by day 4 in either normal or ischemic tissues. We demonstrate that 30-nm aligned nanofibrillar collagen scaffolds guide cellular organization, modulate endothelial inflammatory response, and enhance cell survival after implantation in normal and ischemic tissues.

Original languageEnglish (US)
Pages (from-to)4038-4047
Number of pages10
JournalBiomaterials
Volume34
Issue number16
DOIs
StatePublished - May 2013

Keywords

  • Cell survival
  • Collagen
  • Endothelial cell
  • Extracellular matrix
  • Nanotopography
  • Vascular grafts

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Fingerprint Dive into the research topics of 'The modulation of endothelial cell morphology, function, and survival using anisotropic nanofibrillar collagen scaffolds'. Together they form a unique fingerprint.

Cite this