TY - JOUR
T1 - The metabolism of 4-androstene-3,17-dione by isolated rat hepatocytes. Maintenance of the sex differences in culture
AU - Stenberg, Åke
AU - Skett, Paul
AU - Gustafsson, Jan Åke
N1 - Funding Information:
This work was supported by grants from Karolinska Institutets fonder and from the Swedish Medical Research Council (No. 03X-2819).
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1978/9/28
Y1 - 1978/9/28
N2 - Hepatocytes from adult rats were isolated and cultivated as primary monolayers for 3 days in a medium containing only 1% homologous serum. The metabolism of 4-androstene-3,17-dione was followed in hepatocytes from male, female and hypophysectomized animals. It was found that immediately after preparation, 5α-reductase and 16α-hydroxylase activities were present in the cells in amounts comparable to those found in microsomal preparations from liver homogenates. After 3 days in culture, these enzyme activities had decreased to about half the values measured on day 0. During this time the sexual differences in steroid metabolism in the cells were stable i.e. there was no detectable induction of 16α-hydroxylase in cells from female animals, and the relative sex difference in 5α-reductase activity persisted.
AB - Hepatocytes from adult rats were isolated and cultivated as primary monolayers for 3 days in a medium containing only 1% homologous serum. The metabolism of 4-androstene-3,17-dione was followed in hepatocytes from male, female and hypophysectomized animals. It was found that immediately after preparation, 5α-reductase and 16α-hydroxylase activities were present in the cells in amounts comparable to those found in microsomal preparations from liver homogenates. After 3 days in culture, these enzyme activities had decreased to about half the values measured on day 0. During this time the sexual differences in steroid metabolism in the cells were stable i.e. there was no detectable induction of 16α-hydroxylase in cells from female animals, and the relative sex difference in 5α-reductase activity persisted.
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U2 - 10.1016/0005-2760(78)90161-3
DO - 10.1016/0005-2760(78)90161-3
M3 - Article
C2 - 698241
AN - SCOPUS:0018133956
SN - 0005-2760
VL - 530
SP - 412
EP - 419
JO - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
JF - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
IS - 3
ER -