The metabolism of 4-androstene-3,17-dione by isolated rat hepatocytes. Maintenance of the sex differences in culture

Åke Stenberg, Paul Skett, Jan Åke Gustafsson

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Hepatocytes from adult rats were isolated and cultivated as primary monolayers for 3 days in a medium containing only 1% homologous serum. The metabolism of 4-androstene-3,17-dione was followed in hepatocytes from male, female and hypophysectomized animals. It was found that immediately after preparation, 5α-reductase and 16α-hydroxylase activities were present in the cells in amounts comparable to those found in microsomal preparations from liver homogenates. After 3 days in culture, these enzyme activities had decreased to about half the values measured on day 0. During this time the sexual differences in steroid metabolism in the cells were stable i.e. there was no detectable induction of 16α-hydroxylase in cells from female animals, and the relative sex difference in 5α-reductase activity persisted.

Original languageEnglish (US)
Pages (from-to)412-419
Number of pages8
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume530
Issue number3
DOIs
StatePublished - Sep 28 1978

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

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