The Membrane Proximal Region of the Integrin β Cytoplasmic Domain Can Mediate Oligomerization

Peter E. Zage, Eugene E. Marcantonio

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Integrin-ligand binding generates many intracellular signals, including signals to initiate focal contact formation and to regulate cellular decisions concerning growth and differentiation. Oligomerization of the β subunit cytoplasmic domain appears to be required for many of these events. In order to study these processes, we have generated a novel chimeric protein, consisting of the chicken integrin β1 cytoplasmic domain connected to the central rod domain of a neuronal intermediate filament, α-internexin. This chimeric protein, when expressed transiently in 293T cells, oligomerizes in a β cytoplasmic domain-dependent manner. This oligomerization requires the membrane proximal amino acids LLMII of the β1 cytoplasmic domain, as demonstrated by deletion analysis. Therefore, the integrin β cytoplasmic domain in this system contains an oligomerization function, which may provide some insight as to the function of intact integrins in vivo.

Original languageEnglish (US)
Pages (from-to)335-347
Number of pages13
JournalCell Communication and Adhesion
Volume5
Issue number5
DOIs
StatePublished - 1998

Keywords

  • Cytoplasmic domains
  • Integrin receptors
  • Oligomerization

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry

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