A variable proportion of the total apolipoprotein A-I (apoA-I) present in plasma or high density lipoproteins (HDL) is normally detectable by immunochemical methods. This has been attributed to masking of some of the immunoreactive sites of apo A-I by lipid in the intact HDL particle. This difficulty has been circumvented by heating or delipidation. We find that exposure of plasma to concentrations of urea greater than about 7.0 M in the barbital buffer used to dilute plasma samples for estimation by electroimmunoassay enables the complete detection of ApoA-I, as judged by comparison with samples delipidated with tetramethylurea. The need for time-consuming heating or delipidation is avoided.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of lipid research|
|State||Published - 1980|
ASJC Scopus subject areas
- Cell Biology