The measurement of apolipoprotein A-I in human plasma by electroimmunoassay

J. P. Miller, S. J.T. Mao, J. R. Patsch, Antonio Gotto

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

A variable proportion of the total apolipoprotein A-I (apoA-I) present in plasma or high density lipoproteins (HDL) is normally detectable by immunochemical methods. This has been attributed to masking of some of the immunoreactive sites of apo A-I by lipid in the intact HDL particle. This difficulty has been circumvented by heating or delipidation. We find that exposure of plasma to concentrations of urea greater than about 7.0 M in the barbital buffer used to dilute plasma samples for estimation by electroimmunoassay enables the complete detection of ApoA-I, as judged by comparison with samples delipidated with tetramethylurea. The need for time-consuming heating or delipidation is avoided.

Original languageEnglish (US)
Pages (from-to)775-780
Number of pages6
JournalJournal of lipid research
Volume21
Issue number6
StatePublished - 1980

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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