The maternal store of the xlgv7 mRNA in full-grown oocytes is not required for normal development in Xenopus

M. Kloc, M. Miller, A. E. Carrasco, E. Eastman, L. Etkin

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

We have attempted to analyze the function of a maternal mRNA xlgv7 which is distributed as an animal - vegetal gradient in stage 6 oocytes using a combination of antisense olgiodeoxynucleotide injection into oocytes followed by in vitro maturation and fertilization. Injection of 20 ng of the antisense oligodeoxynucleotide resulted in the destruction of the xlgv7 mRNA to undetectable levels. Upon maturation and fertilization the resulting embryos develop with no specific defects suggesting that the maternal store of xlgv7 in stage 6 oocytes is not required and that the embryo can develop solely with the maternal store of the xlgv7 protein. Also, these results dmeonstrate the feasibility of this approach in destroying a specific maternal RNA and assaying its effect on development.

Original languageEnglish (US)
Pages (from-to)899-907
Number of pages9
JournalDevelopment
Volume107
Issue number4
StatePublished - 1989

Keywords

  • antisense oligo
  • destruction of mRNA
  • maternal messenger RNA
  • oocyte
  • Xenopus laevis

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

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