The intracellular dissipation of cytosolic calcium following glucose re-addition to carbohydrate depleted Saccharomyces cerevisiae

Richard Kellermayer, Réka Szigeti, Miklós Kellermayer, Attila Miseta

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Glucose re-addition to carbohydrate starved yeast cells leads to a transient elevation of eytosolic calcium (TECC). Concomitantly, a cytosolic proton extrusion occurs through the activation of the vacuolar H +-ATPase and the plasma membrane H+-ATPases. This study addressed the dissipation of the TECC through intracellular compartmentalization and the possible affects of the H+-ATPases on this process. Both the vacuole and the Golgi-ER apparatus were found to play important roles in distributing calcium to internal stores. Additionally, the inhibition of cytosolic proton extrusion augmented cytosolic calcium responses. A model where pH dependent cytosolic calcium buffering plays an important role in the dissipation of the TECC in Saccharomyces cerevisiae is proposed.

Original languageEnglish (US)
Pages (from-to)55-60
Number of pages6
JournalFEBS Letters
Volume571
Issue number1-3
DOIs
StatePublished - Jul 30 2004

Keywords

  • Calcium
  • Glucose
  • pH
  • PKC, protein kinase C
  • PLC, phospholipase C
  • PMA, plasma membrane H-ATPases
  • PMR1
  • TECC, transient elevation of cytoplasmic calcium
  • V-ATPase, vacuolar H-ATPase
  • VCX1
  • Yeast

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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