The intracellular dissipation of cytosolic calcium following glucose re-addition to carbohydrate depleted Saccharomyces cerevisiae

Richard Kellermayer; Réka Szigeti; Miklós Kellermayer; Attila Miseta

doi:10.1016/j.febslet.2004.06.049

The intracellular dissipation of cytosolic calcium following glucose re-addition to carbohydrate depleted Saccharomyces cerevisiae

Richard Kellermayer, Réka Szigeti, Miklós Kellermayer, Attila Miseta

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Glucose re-addition to carbohydrate starved yeast cells leads to a transient elevation of eytosolic calcium (TECC). Concomitantly, a cytosolic proton extrusion occurs through the activation of the vacuolar H ⁺-ATPase and the plasma membrane H⁺-ATPases. This study addressed the dissipation of the TECC through intracellular compartmentalization and the possible affects of the H⁺-ATPases on this process. Both the vacuole and the Golgi-ER apparatus were found to play important roles in distributing calcium to internal stores. Additionally, the inhibition of cytosolic proton extrusion augmented cytosolic calcium responses. A model where pH dependent cytosolic calcium buffering plays an important role in the dissipation of the TECC in Saccharomyces cerevisiae is proposed.

Original language	English (US)
Pages (from-to)	55-60
Number of pages	6
Journal	FEBS Letters
Volume	571
Issue number	1-3
DOIs	https://doi.org/10.1016/j.febslet.2004.06.049
State	Published - Jul 30 2004

Keywords

Calcium
Glucose
pH
PKC, protein kinase C
PLC, phospholipase C
PMA, plasma membrane H-ATPases
PMR1
TECC, transient elevation of cytoplasmic calcium
V-ATPase, vacuolar H-ATPase
VCX1
Yeast

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2004.06.049

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title = "The intracellular dissipation of cytosolic calcium following glucose re-addition to carbohydrate depleted Saccharomyces cerevisiae",

abstract = "Glucose re-addition to carbohydrate starved yeast cells leads to a transient elevation of eytosolic calcium (TECC). Concomitantly, a cytosolic proton extrusion occurs through the activation of the vacuolar H +-ATPase and the plasma membrane H+-ATPases. This study addressed the dissipation of the TECC through intracellular compartmentalization and the possible affects of the H+-ATPases on this process. Both the vacuole and the Golgi-ER apparatus were found to play important roles in distributing calcium to internal stores. Additionally, the inhibition of cytosolic proton extrusion augmented cytosolic calcium responses. A model where pH dependent cytosolic calcium buffering plays an important role in the dissipation of the TECC in Saccharomyces cerevisiae is proposed.",

keywords = "Calcium, Glucose, pH, PKC, protein kinase C, PLC, phospholipase C, PMA, plasma membrane H-ATPases, PMR1, TECC, transient elevation of cytoplasmic calcium, V-ATPase, vacuolar H-ATPase, VCX1, Yeast",

author = "Richard Kellermayer and R{\'e}ka Szigeti and Mikl{\'o}s Kellermayer and Attila Miseta",

note = "Funding Information: The authors thank Dr. David Bedwell for providing strains, media and laboratory equipment, Dr. Enzo Martegani and Dr. Martha Cyert for providing strains and plasmids, Dr. David Aiello for fruitful suggestions and Ibolya Lam{\'a}r for her assistance. A.M. was supported by OTKA T-038144, M.K. was supported by OTKA T-032043. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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AU - Szigeti, Réka

AU - Kellermayer, Miklós

AU - Miseta, Attila

N1 - Funding Information: The authors thank Dr. David Bedwell for providing strains, media and laboratory equipment, Dr. Enzo Martegani and Dr. Martha Cyert for providing strains and plasmids, Dr. David Aiello for fruitful suggestions and Ibolya Lamár for her assistance. A.M. was supported by OTKA T-038144, M.K. was supported by OTKA T-032043. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2004/7/30

Y1 - 2004/7/30

N2 - Glucose re-addition to carbohydrate starved yeast cells leads to a transient elevation of eytosolic calcium (TECC). Concomitantly, a cytosolic proton extrusion occurs through the activation of the vacuolar H +-ATPase and the plasma membrane H+-ATPases. This study addressed the dissipation of the TECC through intracellular compartmentalization and the possible affects of the H+-ATPases on this process. Both the vacuole and the Golgi-ER apparatus were found to play important roles in distributing calcium to internal stores. Additionally, the inhibition of cytosolic proton extrusion augmented cytosolic calcium responses. A model where pH dependent cytosolic calcium buffering plays an important role in the dissipation of the TECC in Saccharomyces cerevisiae is proposed.

AB - Glucose re-addition to carbohydrate starved yeast cells leads to a transient elevation of eytosolic calcium (TECC). Concomitantly, a cytosolic proton extrusion occurs through the activation of the vacuolar H +-ATPase and the plasma membrane H+-ATPases. This study addressed the dissipation of the TECC through intracellular compartmentalization and the possible affects of the H+-ATPases on this process. Both the vacuole and the Golgi-ER apparatus were found to play important roles in distributing calcium to internal stores. Additionally, the inhibition of cytosolic proton extrusion augmented cytosolic calcium responses. A model where pH dependent cytosolic calcium buffering plays an important role in the dissipation of the TECC in Saccharomyces cerevisiae is proposed.

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KW - pH

KW - PKC, protein kinase C

KW - PLC, phospholipase C

KW - PMA, plasma membrane H-ATPases

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KW - TECC, transient elevation of cytoplasmic calcium

KW - V-ATPase, vacuolar H-ATPase

KW - VCX1

KW - Yeast

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The intracellular dissipation of cytosolic calcium following glucose re-addition to carbohydrate depleted Saccharomyces cerevisiae

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