The incidence of HIV-1 and HHV-6 in corneal buttons

Hamida B. Qavi; Mary T. Green; Gary K. Segall; F. Blaine Hollinger; Dorothy E. Lewis

doi:10.3109/02713689109020364

The incidence of HIV-1 and HHV-6 in corneal buttons

Hamida B. Qavi, Mary T. Green, Gary K. Segall, F. Blaine Hollinger, Dorothy E. Lewis

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Twenty pairs of corneas from asymptomatic carriers of HIV-1 and seven pairs from AIDS patients were analyzed for the presence of HIV-1 and HHV-6 antigens, viral transcripts, DNA sequences, and intact and infectious particles. Although serum from all donors was positive for both HIV-1 and HHV-6 antibody by Western blot analysis, only one cornea from an asymptomatic carrier of HIV-1 was positive for HIV-1 and HHV-6. The cornea was positive when tested by tissue culture, PCR, in situ hybridization, and electron microscopy. There was no tear film contamination. These results suggest that HIV-1 and HHV-6 may be capable of invading corneal tissue.

Original language	English (US)
Pages (from-to)	97-103
Number of pages	7
Journal	Current Eye Research
Volume	10
Issue number	s1
DOIs	https://doi.org/10.3109/02713689109020364
State	Published - 1991

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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title = "The incidence of HIV-1 and HHV-6 in corneal buttons",

abstract = "Twenty pairs of corneas from asymptomatic carriers of HIV-1 and seven pairs from AIDS patients were analyzed for the presence of HIV-1 and HHV-6 antigens, viral transcripts, DNA sequences, and intact and infectious particles. Although serum from all donors was positive for both HIV-1 and HHV-6 antibody by Western blot analysis, only one cornea from an asymptomatic carrier of HIV-1 was positive for HIV-1 and HHV-6. The cornea was positive when tested by tissue culture, PCR, in situ hybridization, and electron microscopy. There was no tear film contamination. These results suggest that HIV-1 and HHV-6 may be capable of invading corneal tissue.",

author = "Qavi, \{Hamida B.\} and Green, \{Mary T.\} and Segall, \{Gary K.\} and Hollinger, \{F. Blaine\} and Lewis, \{Dorothy E.\}",

note = "Funding Information: ACKNOWLEDGEMENTS This work was supported by a grant from the National Eye Institute (EY08082). The corneas for this study were provided by the Lions Eyes of Texas Eye Bank. We thank Benny Tse and Ed Calomeni for excellent technical assistance. The authors are indebted to Ms. Caroyl Gilbert for typing this manuscript.",

year = "1991",

doi = "10.3109/02713689109020364",

language = "English (US)",

volume = "10",

pages = "97--103",

journal = "Current Eye Research",

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T1 - The incidence of HIV-1 and HHV-6 in corneal buttons

AU - Qavi, Hamida B.

AU - Green, Mary T.

AU - Segall, Gary K.

AU - Hollinger, F. Blaine

AU - Lewis, Dorothy E.

N1 - Funding Information: ACKNOWLEDGEMENTS This work was supported by a grant from the National Eye Institute (EY08082). The corneas for this study were provided by the Lions Eyes of Texas Eye Bank. We thank Benny Tse and Ed Calomeni for excellent technical assistance. The authors are indebted to Ms. Caroyl Gilbert for typing this manuscript.

PY - 1991

Y1 - 1991

N2 - Twenty pairs of corneas from asymptomatic carriers of HIV-1 and seven pairs from AIDS patients were analyzed for the presence of HIV-1 and HHV-6 antigens, viral transcripts, DNA sequences, and intact and infectious particles. Although serum from all donors was positive for both HIV-1 and HHV-6 antibody by Western blot analysis, only one cornea from an asymptomatic carrier of HIV-1 was positive for HIV-1 and HHV-6. The cornea was positive when tested by tissue culture, PCR, in situ hybridization, and electron microscopy. There was no tear film contamination. These results suggest that HIV-1 and HHV-6 may be capable of invading corneal tissue.

AB - Twenty pairs of corneas from asymptomatic carriers of HIV-1 and seven pairs from AIDS patients were analyzed for the presence of HIV-1 and HHV-6 antigens, viral transcripts, DNA sequences, and intact and infectious particles. Although serum from all donors was positive for both HIV-1 and HHV-6 antibody by Western blot analysis, only one cornea from an asymptomatic carrier of HIV-1 was positive for HIV-1 and HHV-6. The cornea was positive when tested by tissue culture, PCR, in situ hybridization, and electron microscopy. There was no tear film contamination. These results suggest that HIV-1 and HHV-6 may be capable of invading corneal tissue.

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The incidence of HIV-1 and HHV-6 in corneal buttons

Abstract

ASJC Scopus subject areas

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