The human genome encodes at least three non-allellic G proteins with αi-type subunits

Wadi N. Suki; Joel Abramowitz; Rafael Mattera; Juan Codina; Lutz Birnbaumer

doi:10.1016/0014-5793(87)80900-6

The human genome encodes at least three non-allellic G proteins with α_i-type subunits

Wadi N. Suki, Joel Abramowitz, Rafael Mattera, Juan Codina, Lutz Birnbaumer

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

The amino acid sequence and composition of α-subunits of signal transducing G proteins of the same kind appear to vary by no more than 2% from species to species. Here we isolated a human liver cDNA using an oligonucleotide complementary to the sequences encoding the pertussis toxin (PTX) ADP-ribosylation site of the α-subunit of the rat brain G protein called G_i. Its open reading frame characterizes it as an α_i-type cDNA-as opposed to α_o-type-but predicts an amino acid composition that differs by 7% and 14%, respectively, from two other human α_i-type molecules. Together with human brain α_i (type-1) and human monocyte α_i (type-2), the new human liver α_i cDNA (type-3) forms part of a family of α_i molecules. Type-3 α_i cDNA hybridizes to a ∼ 3.6 kilobase long mRNA and type-2 α_i cDNA hybridizes to an mRNA species of ∼ 2.7 kilobases. This indicates that the human genome has at least three non-allellic genes encoding non-α_o-type PTX substrates and provides structural evidence for the hypothesis that distinct effector systems are regulated by similar but nevertheless distinct PTX substrates.

Original language	English (US)
Pages (from-to)	187-192
Number of pages	6
Journal	FEBS Letters
Volume	220
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(87)80900-6
State	Published - Aug 10 1987

Keywords

Adenylyl cyclase
G protein
K channel
Pertussis toxin
Phospholipase A
Phospholipase C

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry

Access to Document

10.1016/0014-5793(87)80900-6

Cite this

@article{3bd8754ef6694f8cb459ba1be1fd76b4,

title = "The human genome encodes at least three non-allellic G proteins with αi-type subunits",

abstract = "The amino acid sequence and composition of α-subunits of signal transducing G proteins of the same kind appear to vary by no more than 2% from species to species. Here we isolated a human liver cDNA using an oligonucleotide complementary to the sequences encoding the pertussis toxin (PTX) ADP-ribosylation site of the α-subunit of the rat brain G protein called Gi. Its open reading frame characterizes it as an αi-type cDNA-as opposed to αo-type-but predicts an amino acid composition that differs by 7% and 14%, respectively, from two other human αi-type molecules. Together with human brain αi (type-1) and human monocyte αi (type-2), the new human liver αi cDNA (type-3) forms part of a family of αi molecules. Type-3 αi cDNA hybridizes to a ∼ 3.6 kilobase long mRNA and type-2 αi cDNA hybridizes to an mRNA species of ∼ 2.7 kilobases. This indicates that the human genome has at least three non-allellic genes encoding non-αo-type PTX substrates and provides structural evidence for the hypothesis that distinct effector systems are regulated by similar but nevertheless distinct PTX substrates.",

keywords = "Adenylyl cyclase, G protein, K channel, Pertussis toxin, Phospholipase A, Phospholipase C",

author = "Suki, {Wadi N.} and Joel Abramowitz and Rafael Mattera and Juan Codina and Lutz Birnbaumer",

note = "Funding Information: We thank Drs K. Sullivan and H.R. Bourne, Department of Pharmacology, School of Medicine, University of California at San Francisco for giving us the type-2 murine macrophage at cDNA [lo]. We thank also Dr Savio L.C. Woo (Department of Cell Biology, Baylor College of Medicine) for making available to us the human liver hgtl 1 cDNA library to search for G protein cDNAs. This work was supported in part by NIH research grants DK-19318, . HL-31164 and HD-09581 to L.B., HD-22058 to J.A. and DK-37543 to W.N.S., NIH center grants AM-27685, HD-09495 to Baylor College of Medicine and a Faculty Improvement Leave Grant from Iowa State University to J.A.",

year = "1987",

month = aug,

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doi = "10.1016/0014-5793(87)80900-6",

language = "English (US)",

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T1 - The human genome encodes at least three non-allellic G proteins with αi-type subunits

AU - Suki, Wadi N.

AU - Abramowitz, Joel

AU - Mattera, Rafael

AU - Codina, Juan

AU - Birnbaumer, Lutz

N1 - Funding Information: We thank Drs K. Sullivan and H.R. Bourne, Department of Pharmacology, School of Medicine, University of California at San Francisco for giving us the type-2 murine macrophage at cDNA [lo]. We thank also Dr Savio L.C. Woo (Department of Cell Biology, Baylor College of Medicine) for making available to us the human liver hgtl 1 cDNA library to search for G protein cDNAs. This work was supported in part by NIH research grants DK-19318, . HL-31164 and HD-09581 to L.B., HD-22058 to J.A. and DK-37543 to W.N.S., NIH center grants AM-27685, HD-09495 to Baylor College of Medicine and a Faculty Improvement Leave Grant from Iowa State University to J.A.

PY - 1987/8/10

Y1 - 1987/8/10

N2 - The amino acid sequence and composition of α-subunits of signal transducing G proteins of the same kind appear to vary by no more than 2% from species to species. Here we isolated a human liver cDNA using an oligonucleotide complementary to the sequences encoding the pertussis toxin (PTX) ADP-ribosylation site of the α-subunit of the rat brain G protein called Gi. Its open reading frame characterizes it as an αi-type cDNA-as opposed to αo-type-but predicts an amino acid composition that differs by 7% and 14%, respectively, from two other human αi-type molecules. Together with human brain αi (type-1) and human monocyte αi (type-2), the new human liver αi cDNA (type-3) forms part of a family of αi molecules. Type-3 αi cDNA hybridizes to a ∼ 3.6 kilobase long mRNA and type-2 αi cDNA hybridizes to an mRNA species of ∼ 2.7 kilobases. This indicates that the human genome has at least three non-allellic genes encoding non-αo-type PTX substrates and provides structural evidence for the hypothesis that distinct effector systems are regulated by similar but nevertheless distinct PTX substrates.

AB - The amino acid sequence and composition of α-subunits of signal transducing G proteins of the same kind appear to vary by no more than 2% from species to species. Here we isolated a human liver cDNA using an oligonucleotide complementary to the sequences encoding the pertussis toxin (PTX) ADP-ribosylation site of the α-subunit of the rat brain G protein called Gi. Its open reading frame characterizes it as an αi-type cDNA-as opposed to αo-type-but predicts an amino acid composition that differs by 7% and 14%, respectively, from two other human αi-type molecules. Together with human brain αi (type-1) and human monocyte αi (type-2), the new human liver αi cDNA (type-3) forms part of a family of αi molecules. Type-3 αi cDNA hybridizes to a ∼ 3.6 kilobase long mRNA and type-2 αi cDNA hybridizes to an mRNA species of ∼ 2.7 kilobases. This indicates that the human genome has at least three non-allellic genes encoding non-αo-type PTX substrates and provides structural evidence for the hypothesis that distinct effector systems are regulated by similar but nevertheless distinct PTX substrates.

KW - Adenylyl cyclase

KW - G protein

KW - K channel

KW - Pertussis toxin

KW - Phospholipase A

KW - Phospholipase C

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