The human apolipoprotein B-100 gene: A highly polymorphic gene that maps to the short arm of chromosome 2

L. Chan, P. VanTuinen, D. H. Ledbetter, S. P. Daiger, Antonio Gotto, S. H. Chen

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Using a cloned cDNA of apolipoprotein B-100 as hydridization probe, we have found high frequence polymorphisms in the apoB-100 gene involving sites for the restriction enzymes EcoRI, BamHI, and HindIII. The major EcoRI polymorphisms involved a 17 kb vs 15 kb variant. The incidence of the various phenotypes was estimated. In addition, other complex polymorphisms involving MspI and TaqI sites were also noted. [32P]-labeled apoB-100 cDNA was used as a probe in chromosome mapping studies to detect the human apoB-100 structural gene sequence in human-Chinese hamster and human-mouse cell hybrids. Southern blot analysis of 14 hybrids localized the gene to the short arm of human chromosome 2.

Original languageEnglish (US)
Pages (from-to)248-255
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume133
Issue number1
DOIs
StatePublished - Nov 27 1985

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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