The genomic structure of an insertional mutation in the dystonia musculorum locus

Arthur Brown; Neal G. Copeland; Debra J. Gilbert; Nancy A. Jenkins; Janet Rossant; Rashmi Kothary

doi:10.1006/geno.1994.1190

The genomic structure of an insertional mutation in the dystonia musculorum locus

Arthur Brown, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Janet Rossant, Rashmi Kothary

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32 Scopus citations

Abstract

We have previously identified a line of transgenic mice, Tg4, in which an hsp68-lacZ hybrid gene has inserted into the dystonia musculorum (dt) locus on chromosome 1. We have confirmed the localization of the Tg4 integration site to the proximal region of mouse chromosome 1 by interspecific backcross analysis. One end of the integration complex has been cloned and we have used single-copy probes from the flanking region to screen a mouse genomic library. Several overlapping lambda phage clones have been isolated and arranged into a contig spanning 75 kb of genomic DNA. Probes from the genomic contig have enabled us to characterize the wildtype and Tg4 loci. We report that the integration of the transgene was accompanied by a deletion of 45 kb of host genomic sequences with no other detectable rearrangement in the Tg4 genome.

Original language	English (US)
Pages (from-to)	371-376
Number of pages	6
Journal	Genomics
Volume	20
Issue number	3
DOIs	https://doi.org/10.1006/geno.1994.1190
State	Published - Jan 1 1994

ASJC Scopus subject areas

Genetics

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abstract = "We have previously identified a line of transgenic mice, Tg4, in which an hsp68-lacZ hybrid gene has inserted into the dystonia musculorum (dt) locus on chromosome 1. We have confirmed the localization of the Tg4 integration site to the proximal region of mouse chromosome 1 by interspecific backcross analysis. One end of the integration complex has been cloned and we have used single-copy probes from the flanking region to screen a mouse genomic library. Several overlapping lambda phage clones have been isolated and arranged into a contig spanning 75 kb of genomic DNA. Probes from the genomic contig have enabled us to characterize the wildtype and Tg4 loci. We report that the integration of the transgene was accompanied by a deletion of 45 kb of host genomic sequences with no other detectable rearrangement in the Tg4 genome.",

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AU - Kothary, Rashmi

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AB - We have previously identified a line of transgenic mice, Tg4, in which an hsp68-lacZ hybrid gene has inserted into the dystonia musculorum (dt) locus on chromosome 1. We have confirmed the localization of the Tg4 integration site to the proximal region of mouse chromosome 1 by interspecific backcross analysis. One end of the integration complex has been cloned and we have used single-copy probes from the flanking region to screen a mouse genomic library. Several overlapping lambda phage clones have been isolated and arranged into a contig spanning 75 kb of genomic DNA. Probes from the genomic contig have enabled us to characterize the wildtype and Tg4 loci. We report that the integration of the transgene was accompanied by a deletion of 45 kb of host genomic sequences with no other detectable rearrangement in the Tg4 genome.

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The genomic structure of an insertional mutation in the dystonia musculorum locus

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