The effectiveness of three multi-component binding models in describing the binary competitive equilibrium adsorption of two cytochrome b5 mutants

Tony Cano; Natalie Offringa; Richard C. Willson

doi:10.1016/j.chroma.2007.01.059

The effectiveness of three multi-component binding models in describing the binary competitive equilibrium adsorption of two cytochrome b₅ mutants

Tony Cano, Natalie Offringa, Richard C. Willson

Houston Methodist

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Competitive adsorption isotherms for two conservative surface charge-neutralizing mutants of cytochrome b₅, E11Q and E44Q, previously measured with competitor concentration held constant over the range of the isotherm, were used to test three widely-used multi-component isotherm models. The extended Langmuir-Freundlich, Langmuir and Jovanovic-Freundlich models each adequately described the weaker infinite dilution adsorption of the E44Q protein in the presence of the strong binding E11Q. The extended Langmuir-Freundlich model generally gave the lowest errors at higher concentrations, and the Jovanovic-Freundlich model gave the best fits when using empirically optimized maximal loading values based on multi-component as well as pure-component isotherm data.

Original language	English (US)
Pages (from-to)	197-202
Number of pages	6
Journal	Journal of Chromatography A
Volume	1144
Issue number	2
DOIs	https://doi.org/10.1016/j.chroma.2007.01.059
State	Published - Mar 16 2007

Keywords

Competitive adsorption
Cytochrome
Extended Jovanovic-Freundlich
Extended Langmuir-Freundlich
Ion-exchange
Isotherm
Langmuir

ASJC Scopus subject areas

Analytical Chemistry

Access to Document

10.1016/j.chroma.2007.01.059

Cite this

@article{3e616f70c748458bad4d58b97a9007c1,

title = "The effectiveness of three multi-component binding models in describing the binary competitive equilibrium adsorption of two cytochrome b5 mutants",

abstract = "Competitive adsorption isotherms for two conservative surface charge-neutralizing mutants of cytochrome b5, E11Q and E44Q, previously measured with competitor concentration held constant over the range of the isotherm, were used to test three widely-used multi-component isotherm models. The extended Langmuir-Freundlich, Langmuir and Jovanovic-Freundlich models each adequately described the weaker infinite dilution adsorption of the E44Q protein in the presence of the strong binding E11Q. The extended Langmuir-Freundlich model generally gave the lowest errors at higher concentrations, and the Jovanovic-Freundlich model gave the best fits when using empirically optimized maximal loading values based on multi-component as well as pure-component isotherm data.",

keywords = "Competitive adsorption, Cytochrome, Extended Jovanovic-Freundlich, Extended Langmuir-Freundlich, Ion-exchange, Isotherm, Langmuir",

author = "Tony Cano and Natalie Offringa and Willson, \{Richard C.\}",

note = "Funding Information: This work was funded by the National Science Foundation under grant CTS-0004544 and by the Robert A. Welch Foundation under grant number E-1264. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2007",

month = mar,

day = "16",

doi = "10.1016/j.chroma.2007.01.059",

language = "English (US)",

volume = "1144",

pages = "197--202",

journal = "Journal of Chromatography A",

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T1 - The effectiveness of three multi-component binding models in describing the binary competitive equilibrium adsorption of two cytochrome b5 mutants

AU - Cano, Tony

AU - Offringa, Natalie

AU - Willson, Richard C.

N1 - Funding Information: This work was funded by the National Science Foundation under grant CTS-0004544 and by the Robert A. Welch Foundation under grant number E-1264. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2007/3/16

Y1 - 2007/3/16

N2 - Competitive adsorption isotherms for two conservative surface charge-neutralizing mutants of cytochrome b5, E11Q and E44Q, previously measured with competitor concentration held constant over the range of the isotherm, were used to test three widely-used multi-component isotherm models. The extended Langmuir-Freundlich, Langmuir and Jovanovic-Freundlich models each adequately described the weaker infinite dilution adsorption of the E44Q protein in the presence of the strong binding E11Q. The extended Langmuir-Freundlich model generally gave the lowest errors at higher concentrations, and the Jovanovic-Freundlich model gave the best fits when using empirically optimized maximal loading values based on multi-component as well as pure-component isotherm data.

AB - Competitive adsorption isotherms for two conservative surface charge-neutralizing mutants of cytochrome b5, E11Q and E44Q, previously measured with competitor concentration held constant over the range of the isotherm, were used to test three widely-used multi-component isotherm models. The extended Langmuir-Freundlich, Langmuir and Jovanovic-Freundlich models each adequately described the weaker infinite dilution adsorption of the E44Q protein in the presence of the strong binding E11Q. The extended Langmuir-Freundlich model generally gave the lowest errors at higher concentrations, and the Jovanovic-Freundlich model gave the best fits when using empirically optimized maximal loading values based on multi-component as well as pure-component isotherm data.

KW - Competitive adsorption

KW - Cytochrome

KW - Extended Jovanovic-Freundlich

KW - Extended Langmuir-Freundlich

KW - Ion-exchange

KW - Isotherm

KW - Langmuir

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