TY - JOUR
T1 - The effectiveness of three multi-component binding models in describing the binary competitive equilibrium adsorption of two cytochrome b5 mutants
AU - Cano, Tony
AU - Offringa, Natalie
AU - Willson, Richard C.
N1 - Funding Information:
This work was funded by the National Science Foundation under grant CTS-0004544 and by the Robert A. Welch Foundation under grant number E-1264.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/3/16
Y1 - 2007/3/16
N2 - Competitive adsorption isotherms for two conservative surface charge-neutralizing mutants of cytochrome b5, E11Q and E44Q, previously measured with competitor concentration held constant over the range of the isotherm, were used to test three widely-used multi-component isotherm models. The extended Langmuir-Freundlich, Langmuir and Jovanovic-Freundlich models each adequately described the weaker infinite dilution adsorption of the E44Q protein in the presence of the strong binding E11Q. The extended Langmuir-Freundlich model generally gave the lowest errors at higher concentrations, and the Jovanovic-Freundlich model gave the best fits when using empirically optimized maximal loading values based on multi-component as well as pure-component isotherm data.
AB - Competitive adsorption isotherms for two conservative surface charge-neutralizing mutants of cytochrome b5, E11Q and E44Q, previously measured with competitor concentration held constant over the range of the isotherm, were used to test three widely-used multi-component isotherm models. The extended Langmuir-Freundlich, Langmuir and Jovanovic-Freundlich models each adequately described the weaker infinite dilution adsorption of the E44Q protein in the presence of the strong binding E11Q. The extended Langmuir-Freundlich model generally gave the lowest errors at higher concentrations, and the Jovanovic-Freundlich model gave the best fits when using empirically optimized maximal loading values based on multi-component as well as pure-component isotherm data.
KW - Competitive adsorption
KW - Cytochrome
KW - Extended Jovanovic-Freundlich
KW - Extended Langmuir-Freundlich
KW - Ion-exchange
KW - Isotherm
KW - Langmuir
UR - http://www.scopus.com/inward/record.url?scp=33847022687&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33847022687&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2007.01.059
DO - 10.1016/j.chroma.2007.01.059
M3 - Article
C2 - 17303149
AN - SCOPUS:33847022687
VL - 1144
SP - 197
EP - 202
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 2
ER -