The effectiveness of three multi-component binding models in describing the binary competitive equilibrium adsorption of two cytochrome b5 mutants

Tony Cano, Natalie Offringa, Richard C. Willson

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Competitive adsorption isotherms for two conservative surface charge-neutralizing mutants of cytochrome b5, E11Q and E44Q, previously measured with competitor concentration held constant over the range of the isotherm, were used to test three widely-used multi-component isotherm models. The extended Langmuir-Freundlich, Langmuir and Jovanovic-Freundlich models each adequately described the weaker infinite dilution adsorption of the E44Q protein in the presence of the strong binding E11Q. The extended Langmuir-Freundlich model generally gave the lowest errors at higher concentrations, and the Jovanovic-Freundlich model gave the best fits when using empirically optimized maximal loading values based on multi-component as well as pure-component isotherm data.

Original languageEnglish (US)
Pages (from-to)197-202
Number of pages6
JournalJournal of Chromatography A
Volume1144
Issue number2
DOIs
StatePublished - Mar 16 2007

Keywords

  • Competitive adsorption
  • Cytochrome
  • Extended Jovanovic-Freundlich
  • Extended Langmuir-Freundlich
  • Ion-exchange
  • Isotherm
  • Langmuir

ASJC Scopus subject areas

  • Analytical Chemistry

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