Abstract
Competitive adsorption isotherms for two conservative surface charge-neutralizing mutants of cytochrome b5, E11Q and E44Q, previously measured with competitor concentration held constant over the range of the isotherm, were used to test three widely-used multi-component isotherm models. The extended Langmuir-Freundlich, Langmuir and Jovanovic-Freundlich models each adequately described the weaker infinite dilution adsorption of the E44Q protein in the presence of the strong binding E11Q. The extended Langmuir-Freundlich model generally gave the lowest errors at higher concentrations, and the Jovanovic-Freundlich model gave the best fits when using empirically optimized maximal loading values based on multi-component as well as pure-component isotherm data.
Original language | English (US) |
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Pages (from-to) | 197-202 |
Number of pages | 6 |
Journal | Journal of Chromatography A |
Volume | 1144 |
Issue number | 2 |
DOIs | |
State | Published - Mar 16 2007 |
Keywords
- Competitive adsorption
- Cytochrome
- Extended Jovanovic-Freundlich
- Extended Langmuir-Freundlich
- Ion-exchange
- Isotherm
- Langmuir
ASJC Scopus subject areas
- Analytical Chemistry