Abstract
Using light microscope autoradiography and electron microscopy we studied the effect of juvenile hormone III (JHIII) and β-ecdysone insect molting hormone (β-ecd) on the replication of Tipula iridescent virus (TIV) in suspension cultured cells of Estigmene acrea. JHIII at a concentration of 87.5 μg/ml completely inhibited viral DNA synthesis, but upon removal of JHIII, [3H]thymidine was incorporated into the cytoplasm as detected by autoradiography and virions in developmental stages from the same cell samples were-readily seen by electron microscopy. β-ecd at a concentration of 17.5 μg/ml, unlike JHIII, permitted viral DNA synthesis in the presence of the hormone although at a reduced level when compared to TIV-infected cells. But the presence of β-ecd seemed to prevent capsid formation, although islands similar in fine structure to those of viroplastic centers were seen by electron microscopy. Once β-ecd was removed from the medium, TIV-inoculated cells appeared to synthesize new virions in a normal pattern. Both hormones inhibited host cell DNA synthesis in noninfected cells.
Original language | English (US) |
---|---|
Pages (from-to) | 114-123 |
Number of pages | 10 |
Journal | Journal of Invertebrate Pathology |
Volume | 43 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1984 |
Keywords
- Estigmene acrea
- inhibition
- juvenile hormone
- molting hormone
- Tipula iridescent virus
- viral synthesis
ASJC Scopus subject areas
- Insect Science
- Ecology, Evolution, Behavior and Systematics