The effect of two insect hormones on the replication of Tipula iridescent virus in Estigmene acrea cells in suspension culture

Malgorzata Kloc, Peter E. Lee, S. Tajbakhsh

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Using light microscope autoradiography and electron microscopy we studied the effect of juvenile hormone III (JHIII) and β-ecdysone insect molting hormone (β-ecd) on the replication of Tipula iridescent virus (TIV) in suspension cultured cells of Estigmene acrea. JHIII at a concentration of 87.5 μg/ml completely inhibited viral DNA synthesis, but upon removal of JHIII, [3H]thymidine was incorporated into the cytoplasm as detected by autoradiography and virions in developmental stages from the same cell samples were-readily seen by electron microscopy. β-ecd at a concentration of 17.5 μg/ml, unlike JHIII, permitted viral DNA synthesis in the presence of the hormone although at a reduced level when compared to TIV-infected cells. But the presence of β-ecd seemed to prevent capsid formation, although islands similar in fine structure to those of viroplastic centers were seen by electron microscopy. Once β-ecd was removed from the medium, TIV-inoculated cells appeared to synthesize new virions in a normal pattern. Both hormones inhibited host cell DNA synthesis in noninfected cells.

Original languageEnglish (US)
Pages (from-to)114-123
Number of pages10
JournalJournal of Invertebrate Pathology
Volume43
Issue number1
DOIs
StatePublished - Jan 1984

Keywords

  • Estigmene acrea
  • inhibition
  • juvenile hormone
  • molting hormone
  • Tipula iridescent virus
  • viral synthesis

ASJC Scopus subject areas

  • Insect Science
  • Ecology, Evolution, Behavior and Systematics

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