The effect of temperature on the impedimetric response of bioreceptor hosting hydrogels

Anthony Guiseppi-Elie, Lauren Koch, Stephen H. Finley, Gary E. Wnek

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Biocompatible hydrogels that serve as the hosting membrane for various bioreceptors contribute to the response of impedimetric biosensors. The temperature response of poly(2-hydroxymethacrylate) [p(HEMA)]-based hydrogel networks prepared with poly(ethylene glycol) methacrylate (PEGMA) for enhanced biocompatibility and with N-[tris(hydroxymethyl)methyl] acrylamide (HMMA) was studied. Hydrogels were cross-linked with tetraethyleneglycol diacrylate (TEGDA) and synthesized by UV initiation (2M% DMPA photoinitiator). The p(HEMA-co-PEGMA-co-HMMA) based hydrogels were fabricated as discrete gel pads (D=2.5mm, H=2mm and V=9.82μL) on top of 250μm diameter cysteamine modified and acryloyl (polyethylene glycol)110 N-hydroxy succinamide ester (acryloyl-PEG-NHS) derivatized gold microelectrodes set within 8-well (8W1E) cell culture biochips. Gel pads were fabricated with cross-link densities corresponding to 1, 3, 5, 7, 9 and 12M% TEGDA and were studied by frequency dependent 3-electrode electrochemical impedance spectroscopy (1mHz to 100kHz; 50mV p-t-p) and by temporal 2-electrode impedimetry (64kHz; 50mV p-t-p) over the temperature range 30-45°C at 90% RH or in aqueous 0.1M Tris/KCl at pH 7.2 buffer. The p(HEMA-co-PEGMA-co-HMMA) hydrogels showed an increase in the real component of impedance with increasing cross-link density and demonstrated activation energies for impedimetric transport that ranged from 15kJ/mol (3M%) to 20kJ/mol (12M%) confirming the dominance of proton migration in the impedance of the hydrogels.

Original languageEnglish (US)
Pages (from-to)2275-2280
Number of pages6
JournalBiosensors and Bioelectronics
Issue number5
StatePublished - Jan 15 2011


  • Activation energies
  • Biomimetic
  • Hydrogels
  • Impedance
  • Models
  • Transport

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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