The effect of sodium butyrate on Tipula iridescent virus synthesis in insect suspension cell cultures

Malgorzata Kloc, Peter E. Lee, S. Tajbakhsh, B. D. Matthie

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The effect of sodium butyrate on Tipula iridescent virus (TIV) synthesis in suspension-cultured cells of Estigmene acrea was investigated. Sodium butyrate reduces viral-induced cell fusion but this is reversible with the removal of butyrate. At 7 mM sodium butyrate, TIV replicates in cells within 8 hr, but does not replicate in this time with 10-20 mm butyrate in the cell medium; cells so treated contain large vesicles with inoculum. Upon removal of the inhibitor, TIV replication appears normal, but large inoculum vesicles can still be found in the cytoplasm, and many infected cells have highly condensed chromatin in their nuclei. Sodium butyrate causes a lag of at least 2 hr in viral DNA synthesis as detected by [3H]thymidine incorporation into viroplasmic centres and at 7 mm butyrate viral DNA synthesis is reduced by 50-60%. In comparison, butyrate at 7 and 10 mm concentration does not inhibit host DNA synthesis, but at 15 and 20 mm, nuclear DNA synthesis is markedly reduced.

Original languageEnglish (US)
Pages (from-to)113-123
Number of pages11
JournalJournal of Invertebrate Pathology
Volume42
Issue number1
DOIs
StatePublished - Jul 1983

Keywords

  • Estigmene acrea
  • inhibition
  • sodium butyrate
  • Tipula iridescent virus
  • viral synthesis

ASJC Scopus subject areas

  • Insect Science
  • Ecology, Evolution, Behavior and Systematics

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