The effect of semimature dendritic cell and the levels of Treg on transplantation tolerance of hepatocytes differentiated from mouse embryonic stem cell

Cheng Zhang, Wenwei Liao, Bing Cai, Furorig Liu, Qiong Ke, Xiaofeng Zhu, Xiaoshun He, Anbin Hu

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

To investigate the inducing effect and mechanism of semimature dendritic cell (smDCs) on transplantation tolerance of hepatocytes differentiated from mouse embryonic stem cells (ESCs), and to study the connections between smDCs and regulator}' dendritic cells (regDCs). Methods ESCs of 129 mouse labelled green fluorescent protein (GFP) were induced to hepatocytcs by using previous methods. Meanwhile, bone marrow mononuclear cells of 129 mouse were induced to smDCs and regDCs. Moreover, the hepatocytes differentiated from 129 mouse ESCs were transplanted into liver of BALB/c mouse 3 days after infusing smDCs and regDCs suspension of 129 mouse into BAI.B/c mouse by tail vein respectively. After that, the growth status and survival time of transplanted cells in the recipient and infiltration of lymphocytes in transplant sites were observed. Furthermore, Foxp3 expression of peripheral blood CD4 +T cells was also tested. Results In the control group, the transplanted cells in liver of BALB/c mouse survived only about 1 week. In contrast, the transplanted cells of smDC groups and regDCs groups survived about 4 weeks and the transplant sites of smDC groups also had less CD3 ∗ T cells. The morphology of smDCs were similar with regDCs. The expression of MHC-JI, CD40, CD80 and CD86 on smDCs and regDCs were moderate. Moreover, the Foxp3 expression of peripheral blood CD4 + T cells in smDC groups was higher than that in the control groups, from 1. 11% up to 5.38%. The Foxp3 expression in regDC groups rose to 3. 87% .Conclusion The smDCs could induce transplantation tolerance of hepatocytes differentiated from 129 mouse ESCs in the recipient. The mechanism was associated with high level of Foxp3 ∗ Tregs, which could be increased by means of smDCs appropriate expression of MHC-D, CD40, CD80 and CD86. The smDCs and regDCs were the same type of tolerance dendritic cells.

Original languageEnglish (US)
Pages (from-to)259-264
Number of pages6
JournalNational Medical Journal of China
Volume97
Issue number4
DOIs
StatePublished - Jan 24 2017

Keywords

  • Embryonic stem cells
  • Hepatocyte transplantation
  • Rregulatory dendritic cells
  • Semimature dendritic cells
  • Tregs

ASJC Scopus subject areas

  • Medicine(all)

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