TY - JOUR
T1 - The effect of semimature dendritic cell and the levels of Treg on transplantation tolerance of hepatocytes differentiated from mouse embryonic stem cell
AU - Zhang, Cheng
AU - Liao, Wenwei
AU - Cai, Bing
AU - Liu, Furorig
AU - Ke, Qiong
AU - Zhu, Xiaofeng
AU - He, Xiaoshun
AU - Hu, Anbin
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/1/24
Y1 - 2017/1/24
N2 - To investigate the inducing effect and mechanism of semimature dendritic cell (smDCs) on transplantation tolerance of hepatocytes differentiated from mouse embryonic stem cells (ESCs), and to study the connections between smDCs and regulator}' dendritic cells (regDCs). Methods ESCs of 129 mouse labelled green fluorescent protein (GFP) were induced to hepatocytcs by using previous methods. Meanwhile, bone marrow mononuclear cells of 129 mouse were induced to smDCs and regDCs. Moreover, the hepatocytes differentiated from 129 mouse ESCs were transplanted into liver of BALB/c mouse 3 days after infusing smDCs and regDCs suspension of 129 mouse into BAI.B/c mouse by tail vein respectively. After that, the growth status and survival time of transplanted cells in the recipient and infiltration of lymphocytes in transplant sites were observed. Furthermore, Foxp3 expression of peripheral blood CD4 +T cells was also tested. Results In the control group, the transplanted cells in liver of BALB/c mouse survived only about 1 week. In contrast, the transplanted cells of smDC groups and regDCs groups survived about 4 weeks and the transplant sites of smDC groups also had less CD3 ∗ T cells. The morphology of smDCs were similar with regDCs. The expression of MHC-JI, CD40, CD80 and CD86 on smDCs and regDCs were moderate. Moreover, the Foxp3 expression of peripheral blood CD4 + T cells in smDC groups was higher than that in the control groups, from 1. 11% up to 5.38%. The Foxp3 expression in regDC groups rose to 3. 87% .Conclusion The smDCs could induce transplantation tolerance of hepatocytes differentiated from 129 mouse ESCs in the recipient. The mechanism was associated with high level of Foxp3 ∗ Tregs, which could be increased by means of smDCs appropriate expression of MHC-D, CD40, CD80 and CD86. The smDCs and regDCs were the same type of tolerance dendritic cells.
AB - To investigate the inducing effect and mechanism of semimature dendritic cell (smDCs) on transplantation tolerance of hepatocytes differentiated from mouse embryonic stem cells (ESCs), and to study the connections between smDCs and regulator}' dendritic cells (regDCs). Methods ESCs of 129 mouse labelled green fluorescent protein (GFP) were induced to hepatocytcs by using previous methods. Meanwhile, bone marrow mononuclear cells of 129 mouse were induced to smDCs and regDCs. Moreover, the hepatocytes differentiated from 129 mouse ESCs were transplanted into liver of BALB/c mouse 3 days after infusing smDCs and regDCs suspension of 129 mouse into BAI.B/c mouse by tail vein respectively. After that, the growth status and survival time of transplanted cells in the recipient and infiltration of lymphocytes in transplant sites were observed. Furthermore, Foxp3 expression of peripheral blood CD4 +T cells was also tested. Results In the control group, the transplanted cells in liver of BALB/c mouse survived only about 1 week. In contrast, the transplanted cells of smDC groups and regDCs groups survived about 4 weeks and the transplant sites of smDC groups also had less CD3 ∗ T cells. The morphology of smDCs were similar with regDCs. The expression of MHC-JI, CD40, CD80 and CD86 on smDCs and regDCs were moderate. Moreover, the Foxp3 expression of peripheral blood CD4 + T cells in smDC groups was higher than that in the control groups, from 1. 11% up to 5.38%. The Foxp3 expression in regDC groups rose to 3. 87% .Conclusion The smDCs could induce transplantation tolerance of hepatocytes differentiated from 129 mouse ESCs in the recipient. The mechanism was associated with high level of Foxp3 ∗ Tregs, which could be increased by means of smDCs appropriate expression of MHC-D, CD40, CD80 and CD86. The smDCs and regDCs were the same type of tolerance dendritic cells.
KW - Embryonic stem cells
KW - Hepatocyte transplantation
KW - Rregulatory dendritic cells
KW - Semimature dendritic cells
KW - Tregs
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U2 - 10.3760/cma.j.issn.0376-2491.2017.04.005
DO - 10.3760/cma.j.issn.0376-2491.2017.04.005
M3 - Article
C2 - 28162154
AN - SCOPUS:85014050336
SN - 0376-2491
VL - 97
SP - 259
EP - 264
JO - National Medical Journal of China
JF - National Medical Journal of China
IS - 4
ER -