The effect of 7H-dibenzo[c, g]carbazole and benzo[a]pyrene mixture on DNA adduct formation in strain A/J mouse model: Preliminary report

Angela Siner, Weiling Xue, Glenn Talaska, David Warshawsky, Deborah L. Gray, Ronald Nines, Yian Wang, Ruisheng Yao, Gary D. Stoner

Research output: Contribution to journalArticlepeer-review

Abstract

Complex mixtures consist of homocyclic and heterocyclic polycyclic aromatic compounds (PACs) represented by benzo[a]pyrene (BaP) and 7H-dibenzo[c,g]carbazole (DBC), respectively. To exert their biological effects, PACs are metabolized into reactive intermediates, which can form DNA adducts. In this preliminary report, male A/J mice were given a single intraperitoneal injection. Groups of three animals were treated with DBC (2 or 10 mg/kg) or BaP (10 or 100 mg/kg). Mixtures of DBC:BaP were given at doses o 2:10, 2:100, 10:10, or 10:100 mg/kg. DNA adduct levels in lungs collected three days posttreatment were determined by the 32P-postlabeling method. The results indicate that, in the lungs, exposure to mixtures containing more BaP than DBC resulted in the absence of adduct 3 (DBC) and significantly higher total adduct levels. This suggests that BaP is being preferentially metabolized, resulting in less DBC adduction.

Original languageEnglish (US)
Pages (from-to)893-897
Number of pages5
JournalPolycyclic Aromatic Compounds
Volume22
Issue number3-4
DOIs
StatePublished - 2002

Keywords

  • 7H-dibenzo[c,g]carbazole
  • Benzo[a]pyrene
  • DNA adducts
  • Mixtures
  • Polycyclic aromatic compounds (PACs)
  • Strain A/J

ASJC Scopus subject areas

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

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