The distribution of oxidatively-modified lysine in the human vasculature

Gregory D. Sloop, Kenneth B. Fallon, Gary Lipscomb, Hidehiro Takei, Art Zieske

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Fifty-seven sections of human vessels, collected in the Pathobiological Determinants of Atherosclerosis in Youth study from individuals aged 25-34, were stained with two monoclonal antibodies to oxidatively-modified lysine. Intensity and extent of immunoreactivity were graded by three pathologists. Aorta from a Watanabe heritable hyperlipidemic (WHHL) rabbit was stained as a positive control. Intimal immunoreactivity in the rabbit was predominantly localized to lesions. Although immunoreactivity in humans was somewhat more intense in atherosclerotic plaques, substantial staining was present in intima with diffuse intimal thickening and coronary veins. Localization of oxidatively-modified lysine in humans did not correlate with localization or severity of atherosclerosis. Localization of immunoreactivity for oxidatively-modified lysine to intimal lesions in the WHHL rabbit may be due to absence of diffuse intimal thickening, which prevents retention of epitopes throughout the intima. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)255-263
Number of pages9
JournalAtherosclerosis
Volume148
Issue number2
DOIs
StatePublished - Feb 1 2000

Keywords

  • Atherosclerosis
  • Diffuse intimal thickening
  • Oxidized LDL
  • Watanabe heritable hyperlipidemic rabbit

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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