TY - JOUR
T1 - The dioxin receptor
T2 - A comparison with the glucocorticoid receptor
AU - Cuthill, Scott
AU - Wilhelmsson, Anna
AU - Mason, Grant G.F.
AU - Gillner, Mikael
AU - Poellinger, Lorenz
AU - Gustafsson, Jan Åke
N1 - Funding Information:
Acknowledgements-This work was supported by a grant from the Swedish Cancer Society and by Swedish Medical Research Council Grant 13X-2819, and National Institutes of Health Grant ES0 3954-01.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1988
Y1 - 1988
N2 - The physico-chemical properties of the dioxin and glucocorticoid receptors from rat liver and wild-type and mutant cell lines were investigated and compared. In rat liver, the receptors are virtually indistinguishable. Both are highly asymmetrical proteins with axial ratios of 12-15, have Stokes radii of 6 nm and sedimentation coefficients of ~4 S. This results in a calculated apparent mol. wt of ~ 100,000. The dioxin receptor from the mouse hepatoma cell line Hepa lclc7 represents an atypical form of the dioxin receptor with a pronounced tendency to aggregate to form Mr ≈ 300,000 complexes in high ionic strength and in the absence of sodium molybdate. In the presence of sulphydryl reducing agents, however, the Hepa lclc7 dioxin receptor dissociates to an Mr ≈ 100,000 species. In analogy to the nt- mutant glucocorticoid receptor in mouse lymphoma cells, there is no gross change in the structure of the nt- dioxin mutant in mouse hepatoma cells compared with the wild-type receptor. The nt~ dioxin receptor does, however, have a reduced affinity for DNA.
AB - The physico-chemical properties of the dioxin and glucocorticoid receptors from rat liver and wild-type and mutant cell lines were investigated and compared. In rat liver, the receptors are virtually indistinguishable. Both are highly asymmetrical proteins with axial ratios of 12-15, have Stokes radii of 6 nm and sedimentation coefficients of ~4 S. This results in a calculated apparent mol. wt of ~ 100,000. The dioxin receptor from the mouse hepatoma cell line Hepa lclc7 represents an atypical form of the dioxin receptor with a pronounced tendency to aggregate to form Mr ≈ 300,000 complexes in high ionic strength and in the absence of sodium molybdate. In the presence of sulphydryl reducing agents, however, the Hepa lclc7 dioxin receptor dissociates to an Mr ≈ 100,000 species. In analogy to the nt- mutant glucocorticoid receptor in mouse lymphoma cells, there is no gross change in the structure of the nt- dioxin mutant in mouse hepatoma cells compared with the wild-type receptor. The nt~ dioxin receptor does, however, have a reduced affinity for DNA.
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U2 - 10.1016/0022-4731(88)90106-9
DO - 10.1016/0022-4731(88)90106-9
M3 - Article
C2 - 3386253
AN - SCOPUS:0023678404
SN - 0022-4731
VL - 30
SP - 277
EP - 280
JO - Journal of Steroid Biochemistry
JF - Journal of Steroid Biochemistry
IS - 1-6
ER -