The detection of enzyme induction by rat liver microsomes prepared by isoelectric precipitation

ANDREW PARKINSON, STEPHEN SAFE

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

A comparison was made of various indices of the hepatic drug‐metabolizing apparatus associated with the postmitochondrial supernatant, microsomes harvested by differential centrifugation, and microsomes harvested by isoelectric precipitation from control, phenobarbitone‐pretreated and 3‐methylcholanthrene‐pretreated rats. The metabolic capabilities and distinctions between control and induced rats for each of the three liver preparations compared favourably as determined by the concentration of cytochrome b5 and P‐450 and by the activity of NADPH‐cytochrome c reductase, 4‐dimethylaminoantipyrine N‐demethylase and aryl hydrocarbon (benzo[a]pyrene) hydroxylase. The results suggest that the relatively simple isoelectric precipitation technique is a useful alternative to the conventional method of differential centrifugation for the preparation of hepatic microsomes. 1979 Royal Pharmaceutical Society of Great Britain

Original languageEnglish (US)
Pages (from-to)444-447
Number of pages4
JournalJournal of Pharmacy and Pharmacology
Volume31
Issue number1
DOIs
StatePublished - Sep 1979

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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