TY - JOUR
T1 - The detection of enzyme induction by rat liver microsomes prepared by isoelectric precipitation
AU - PARKINSON, ANDREW
AU - SAFE, STEPHEN
PY - 1979/9
Y1 - 1979/9
N2 - A comparison was made of various indices of the hepatic drug‐metabolizing apparatus associated with the postmitochondrial supernatant, microsomes harvested by differential centrifugation, and microsomes harvested by isoelectric precipitation from control, phenobarbitone‐pretreated and 3‐methylcholanthrene‐pretreated rats. The metabolic capabilities and distinctions between control and induced rats for each of the three liver preparations compared favourably as determined by the concentration of cytochrome b5 and P‐450 and by the activity of NADPH‐cytochrome c reductase, 4‐dimethylaminoantipyrine N‐demethylase and aryl hydrocarbon (benzo[a]pyrene) hydroxylase. The results suggest that the relatively simple isoelectric precipitation technique is a useful alternative to the conventional method of differential centrifugation for the preparation of hepatic microsomes. 1979 Royal Pharmaceutical Society of Great Britain
AB - A comparison was made of various indices of the hepatic drug‐metabolizing apparatus associated with the postmitochondrial supernatant, microsomes harvested by differential centrifugation, and microsomes harvested by isoelectric precipitation from control, phenobarbitone‐pretreated and 3‐methylcholanthrene‐pretreated rats. The metabolic capabilities and distinctions between control and induced rats for each of the three liver preparations compared favourably as determined by the concentration of cytochrome b5 and P‐450 and by the activity of NADPH‐cytochrome c reductase, 4‐dimethylaminoantipyrine N‐demethylase and aryl hydrocarbon (benzo[a]pyrene) hydroxylase. The results suggest that the relatively simple isoelectric precipitation technique is a useful alternative to the conventional method of differential centrifugation for the preparation of hepatic microsomes. 1979 Royal Pharmaceutical Society of Great Britain
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U2 - 10.1111/j.2042-7158.1979.tb13550.x
DO - 10.1111/j.2042-7158.1979.tb13550.x
M3 - Article
C2 - 38312
AN - SCOPUS:0018384287
SN - 0022-3573
VL - 31
SP - 444
EP - 447
JO - Journal of Pharmacy and Pharmacology
JF - Journal of Pharmacy and Pharmacology
IS - 1
ER -