The design and fabrication of a three-dimensional bioengineered open ventricle

Nikita M. Patel; Mohamed A. Mohamed; Iman K. Yazdi; Ennio Tasciotti; Ravi K. Birla

doi:10.1002/jbm.b.33742

The design and fabrication of a three-dimensional bioengineered open ventricle

Nikita M. Patel, Mohamed A. Mohamed, Iman K. Yazdi, Ennio Tasciotti, Ravi K. Birla

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Current treatments in hypoplastic left heart syndrome (HLHS) include multiple surgeries to refunctionalize the right ventricle and/or transplant. The development of a tissue-engineered left ventricle (LV) would provide a therapeutic option to overcome the inefficiencies and limitations associated with current treatment options. This study provides a foundation for the development and fabrication of the bioengineered open ventricle (BEOV) model. BEOV molds were developed to emulate the human LV geometry; molds were used to produce chitosan scaffolds. BEOV were fabricated by culturing 30 million rat neonatal cardiac cells on the chitosan scaffold. The model demonstrated 57% cell retention following 4days culture. The average biopotential output for the model was 1615 µV. Histological assessment displayed the presence of localized cell clusters, with intercellular and cell-scaffold interactions. The BEOV provides a novel foundation for the development of a 3D bioengineered LV for application in HLHS.

Original language	English (US)
Pages (from-to)	2206-2217
Number of pages	12
Journal	Journal of Biomedical Materials Research - Part B Applied Biomaterials
Volume	105
Issue number	8
DOIs	https://doi.org/10.1002/jbm.b.33742
State	Published - Nov 2017

Keywords

biocompatibility
cardiac tissue engineering
chitosan
left ventricle
material characterization

ASJC Scopus subject areas

Biomaterials
Biomedical Engineering

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10.1002/jbm.b.33742

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title = "The design and fabrication of a three-dimensional bioengineered open ventricle",

abstract = "Current treatments in hypoplastic left heart syndrome (HLHS) include multiple surgeries to refunctionalize the right ventricle and/or transplant. The development of a tissue-engineered left ventricle (LV) would provide a therapeutic option to overcome the inefficiencies and limitations associated with current treatment options. This study provides a foundation for the development and fabrication of the bioengineered open ventricle (BEOV) model. BEOV molds were developed to emulate the human LV geometry; molds were used to produce chitosan scaffolds. BEOV were fabricated by culturing 30 million rat neonatal cardiac cells on the chitosan scaffold. The model demonstrated 57% cell retention following 4days culture. The average biopotential output for the model was 1615 µV. Histological assessment displayed the presence of localized cell clusters, with intercellular and cell-scaffold interactions. The BEOV provides a novel foundation for the development of a 3D bioengineered LV for application in HLHS.",

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author = "Patel, {Nikita M.} and Mohamed, {Mohamed A.} and Yazdi, {Iman K.} and Ennio Tasciotti and Birla, {Ravi K.}",

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AU - Birla, Ravi K.

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AB - Current treatments in hypoplastic left heart syndrome (HLHS) include multiple surgeries to refunctionalize the right ventricle and/or transplant. The development of a tissue-engineered left ventricle (LV) would provide a therapeutic option to overcome the inefficiencies and limitations associated with current treatment options. This study provides a foundation for the development and fabrication of the bioengineered open ventricle (BEOV) model. BEOV molds were developed to emulate the human LV geometry; molds were used to produce chitosan scaffolds. BEOV were fabricated by culturing 30 million rat neonatal cardiac cells on the chitosan scaffold. The model demonstrated 57% cell retention following 4days culture. The average biopotential output for the model was 1615 µV. Histological assessment displayed the presence of localized cell clusters, with intercellular and cell-scaffold interactions. The BEOV provides a novel foundation for the development of a 3D bioengineered LV for application in HLHS.

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The design and fabrication of a three-dimensional bioengineered open ventricle

Abstract

Keywords

ASJC Scopus subject areas

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