The design and fabrication of a three-dimensional bioengineered open ventricle

Nikita M. Patel, Mohamed A. Mohamed, Iman K. Yazdi, Ennio Tasciotti, Ravi K. Birla

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Current treatments in hypoplastic left heart syndrome (HLHS) include multiple surgeries to refunctionalize the right ventricle and/or transplant. The development of a tissue-engineered left ventricle (LV) would provide a therapeutic option to overcome the inefficiencies and limitations associated with current treatment options. This study provides a foundation for the development and fabrication of the bioengineered open ventricle (BEOV) model. BEOV molds were developed to emulate the human LV geometry; molds were used to produce chitosan scaffolds. BEOV were fabricated by culturing 30 million rat neonatal cardiac cells on the chitosan scaffold. The model demonstrated 57% cell retention following 4days culture. The average biopotential output for the model was 1615 µV. Histological assessment displayed the presence of localized cell clusters, with intercellular and cell-scaffold interactions. The BEOV provides a novel foundation for the development of a 3D bioengineered LV for application in HLHS.

Original languageEnglish (US)
Pages (from-to)2206-2217
Number of pages12
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume105
Issue number8
DOIs
StatePublished - Nov 2017

Keywords

  • biocompatibility
  • cardiac tissue engineering
  • chitosan
  • left ventricle
  • material characterization

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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