Abstract
Current treatments in hypoplastic left heart syndrome (HLHS) include multiple surgeries to refunctionalize the right ventricle and/or transplant. The development of a tissue-engineered left ventricle (LV) would provide a therapeutic option to overcome the inefficiencies and limitations associated with current treatment options. This study provides a foundation for the development and fabrication of the bioengineered open ventricle (BEOV) model. BEOV molds were developed to emulate the human LV geometry; molds were used to produce chitosan scaffolds. BEOV were fabricated by culturing 30 million rat neonatal cardiac cells on the chitosan scaffold. The model demonstrated 57% cell retention following 4days culture. The average biopotential output for the model was 1615 µV. Histological assessment displayed the presence of localized cell clusters, with intercellular and cell-scaffold interactions. The BEOV provides a novel foundation for the development of a 3D bioengineered LV for application in HLHS.
Original language | English (US) |
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Pages (from-to) | 2206-2217 |
Number of pages | 12 |
Journal | Journal of Biomedical Materials Research - Part B Applied Biomaterials |
Volume | 105 |
Issue number | 8 |
DOIs | |
State | Published - Nov 2017 |
Keywords
- biocompatibility
- cardiac tissue engineering
- chitosan
- left ventricle
- material characterization
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering