The design and fabrication of a three-dimensional bioengineered open ventricle

Nikita M. Patel; Mohamed A. Mohamed; Iman K. Yazdi; Ennio Tasciotti; Ravi K. Birla

doi:10.1002/jbm.b.33742

The design and fabrication of a three-dimensional bioengineered open ventricle

Nikita M. Patel, Mohamed A. Mohamed, Iman K. Yazdi, Ennio Tasciotti, Ravi K. Birla

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Current treatments in hypoplastic left heart syndrome (HLHS) include multiple surgeries to refunctionalize the right ventricle and/or transplant. The development of a tissue-engineered left ventricle (LV) would provide a therapeutic option to overcome the inefficiencies and limitations associated with current treatment options. This study provides a foundation for the development and fabrication of the bioengineered open ventricle (BEOV) model. BEOV molds were developed to emulate the human LV geometry; molds were used to produce chitosan scaffolds. BEOV were fabricated by culturing 30 million rat neonatal cardiac cells on the chitosan scaffold. The model demonstrated 57% cell retention following 4days culture. The average biopotential output for the model was 1615 µV. Histological assessment displayed the presence of localized cell clusters, with intercellular and cell-scaffold interactions. The BEOV provides a novel foundation for the development of a 3D bioengineered LV for application in HLHS.

Original language	English (US)
Pages (from-to)	2206-2217
Number of pages	12
Journal	Journal of Biomedical Materials Research - Part B Applied Biomaterials
Volume	105
Issue number	8
DOIs	https://doi.org/10.1002/jbm.b.33742
State	Published - Nov 2017

Keywords

biocompatibility
cardiac tissue engineering
chitosan
left ventricle
material characterization

ASJC Scopus subject areas

Biomaterials
Biomedical Engineering

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10.1002/jbm.b.33742

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title = "The design and fabrication of a three-dimensional bioengineered open ventricle",

abstract = "Current treatments in hypoplastic left heart syndrome (HLHS) include multiple surgeries to refunctionalize the right ventricle and/or transplant. The development of a tissue-engineered left ventricle (LV) would provide a therapeutic option to overcome the inefficiencies and limitations associated with current treatment options. This study provides a foundation for the development and fabrication of the bioengineered open ventricle (BEOV) model. BEOV molds were developed to emulate the human LV geometry; molds were used to produce chitosan scaffolds. BEOV were fabricated by culturing 30 million rat neonatal cardiac cells on the chitosan scaffold. The model demonstrated 57% cell retention following 4days culture. The average biopotential output for the model was 1615 µV. Histological assessment displayed the presence of localized cell clusters, with intercellular and cell-scaffold interactions. The BEOV provides a novel foundation for the development of a 3D bioengineered LV for application in HLHS.",

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author = "Patel, {Nikita M.} and Mohamed, {Mohamed A.} and Yazdi, {Iman K.} and Ennio Tasciotti and Birla, {Ravi K.}",

note = "Funding Information: The researchers would like to acknowledge NIH for provision of funding for this research (Grant number: R01-EB011516). We would also like to thank the Department of Biomedical Engineering and the Cullen College of Engineering at University of Houston for further financial support. We'd like to thank George J. and Angelina P. Kostas Charitable Foundation, Brown Foundation Inc., William Randolph Hearst Foundation, and The Regenerative Medicine Program Cullen Trust for Healthcare for their support of Ennio Tasciotti and Iman Yazdi. Publisher Copyright: {\textcopyright} 2016 Wiley Periodicals, Inc. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

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N1 - Funding Information: The researchers would like to acknowledge NIH for provision of funding for this research (Grant number: R01-EB011516). We would also like to thank the Department of Biomedical Engineering and the Cullen College of Engineering at University of Houston for further financial support. We'd like to thank George J. and Angelina P. Kostas Charitable Foundation, Brown Foundation Inc., William Randolph Hearst Foundation, and The Regenerative Medicine Program Cullen Trust for Healthcare for their support of Ennio Tasciotti and Iman Yazdi. Publisher Copyright: © 2016 Wiley Periodicals, Inc. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

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N2 - Current treatments in hypoplastic left heart syndrome (HLHS) include multiple surgeries to refunctionalize the right ventricle and/or transplant. The development of a tissue-engineered left ventricle (LV) would provide a therapeutic option to overcome the inefficiencies and limitations associated with current treatment options. This study provides a foundation for the development and fabrication of the bioengineered open ventricle (BEOV) model. BEOV molds were developed to emulate the human LV geometry; molds were used to produce chitosan scaffolds. BEOV were fabricated by culturing 30 million rat neonatal cardiac cells on the chitosan scaffold. The model demonstrated 57% cell retention following 4days culture. The average biopotential output for the model was 1615 µV. Histological assessment displayed the presence of localized cell clusters, with intercellular and cell-scaffold interactions. The BEOV provides a novel foundation for the development of a 3D bioengineered LV for application in HLHS.

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The design and fabrication of a three-dimensional bioengineered open ventricle

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Keywords

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