The Characteristics of Naive-like T Cells in Tumor-infiltrating Lymphocytes from Human Lung Cancer

Si Yuan Sheng, Yong Gu, Chuan Gang Lu, Ying Ying Tang, Jian Yong Zou, Yu Qing Zhang, Rong Fu Wang, Hai Hong

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Adoptive cell therapy using autologous tumorinfiltrating lymphocytes (TILs) or genetically modified lymphocytes from TILs is a new effective approach, but the application of TIL immunotherapy is still limited in many solid tumors. Knowledge of the classification and function of TILs is important to develop personalized immunotherapy with TILs in non-small lung cancer (NSCLC). In this study, we show the characteristics of T-cell subsets in TILs isolated from NSCLC. CD3+ CD8+ CD45RA+ T cells outnumbered CD3+ CD4+ CD45RA+ T cells in CD45RA+ TILs, but it was the opposite in CD45RO+ TILs. Effector memory CD4+ T cells predominated in CD4+ TILs; about 10% of the stem cell-like memory T cells (Tscm) were detected in TILs. To further analyze their functions, we stimulated TILs from NSCLC patients by mitogens to examine cytokine production. Our data demonstrated that naive-phenotype T cells in TILs secret IFN-g in abundance; TNF-α-producing T cells were significantly increased in TILs; there were more IL-17-expressing CD4+ Tscm cells than other subtypes of CD4+T cells in TILs. Our findings indicate that the CD4+/CD8+ naive-phenotype T cells and Tscm cells in TILs from lung cancer exhibit distinct composition and strong cytokine production. Attributes of Tscm cells from a naive-like T-cell population in TILs are the promising cell type for adoptive cell therapy in human lung cancer.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalJournal of Immunotherapy
Volume40
Issue number1
DOIs
StatePublished - 2017

Keywords

  • T cell
  • lung cancer
  • tumor-infiltrating lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology
  • Cancer Research

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