The association of silicon microparticles with endothelial cells in drug delivery to the vasculature

Rita Serda, Jianhua Gu, Rohan C. Bhavane, Xuewu Liu, Ciro Chiappini, Paolo Decuzzi, Mauro Ferrari

Research output: Contribution to journalArticle

139 Scopus citations

Abstract

Endothelial targeting is an approach evolving for drug delivery to the vasculature of pathological lesions. Nano-porous silicon-based multi-functional particles are of particular interest, since they can be manufactured in essentially any size and shape, employing methods of photolithography, to optimize their ability to localize on target endothelia. In this study we tested the impact of surface charge, serum opsonization, and inflammation on the ability of vascular endothelial cells to associate with nano-porous silicon microparticles. Vascular endothelial cells were capable of rapidly internalizing both positive and negative silicon microparticles by an actin-dependent mechanism involving both phagocytosis and macropinocytosis. However, following serum opsonization, internalization was selective for APTES (originally positive) modified microparticles, despite the finding that all opsonized microparticles had a net negative charge. Conversely, macrophages displayed a preference for internalization of serum opsonized oxidized (originally negative) microparticles, supporting the choice of positive microparticles for endothelial targeting. The internalization of opsonized microparticles by endothelial cells was further enhanced by the presence of inflammatory cytokines. These findings suggest that it may be possible to bioengineer silicon microparticles to favor opsonization with proteins that enhance uptake by endothelial cells, without a concurrent enhanced uptake by macrophages.

Original languageEnglish (US)
Pages (from-to)2440-2448
Number of pages9
JournalBiomaterials
Volume30
Issue number13
DOIs
StatePublished - May 2009

Keywords

  • Endothelia
  • Microparticles
  • Phagocytosis
  • Silicon

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Fingerprint Dive into the research topics of 'The association of silicon microparticles with endothelial cells in drug delivery to the vasculature'. Together they form a unique fingerprint.

Cite this