TY - JOUR
T1 - The accurate localization of fluorescent nanoparticle Au - Ft in nu/nu mice kidney
AU - Yang, Hui
AU - Sun, Cuiji
AU - Jia, Jun
AU - Nie, Guangjun
AU - Wu, Zhenyu
N1 - Funding Information:
This work was supported by NSFC (31000452; 30900278). H. Y. Gratefully acknowledges the support of Chilong Healthcare International Limited (Beijing) on the using of Nuance multispectral imaging system (CRi, Woburn, MA).
Publisher Copyright:
© 2014 The Authors.
PY - 2014
Y1 - 2014
N2 - Au-Ft, as a green synthesized nanoparticle, is composed of a ferritin nanocage enclosing a pair of Au nanoclusters inside. Our previous study has demonstrated that Au-Ft can be an excellent fluorescent probe for whole body imaging of mice with kidney specific targeting. But, the accurate localization of Au-Ft in kidney is still absent. In the current study, we detected and assessed the cellular and subcellular localization of Au-Ft in renal cortex and medulla of nu/nu mice after tail vein injection by using Nuance optical system (CRi, Woburn, USA) and inForm intelligent image analysis software based on single cell segmentation. We obtained the fluorescence intensity and cellular location of kidney-targeting Au-Ft probe in particular cell of renal glomerulus or renal tubules, which provided valuable proofs to clarify the mechanism of Au-Ft selective enrichment in kidney and the associated metabolic processes.
AB - Au-Ft, as a green synthesized nanoparticle, is composed of a ferritin nanocage enclosing a pair of Au nanoclusters inside. Our previous study has demonstrated that Au-Ft can be an excellent fluorescent probe for whole body imaging of mice with kidney specific targeting. But, the accurate localization of Au-Ft in kidney is still absent. In the current study, we detected and assessed the cellular and subcellular localization of Au-Ft in renal cortex and medulla of nu/nu mice after tail vein injection by using Nuance optical system (CRi, Woburn, USA) and inForm intelligent image analysis software based on single cell segmentation. We obtained the fluorescence intensity and cellular location of kidney-targeting Au-Ft probe in particular cell of renal glomerulus or renal tubules, which provided valuable proofs to clarify the mechanism of Au-Ft selective enrichment in kidney and the associated metabolic processes.
KW - Au-Ft nanoparticle
KW - cellular localization
KW - kidney targeting
KW - multispectral imaging
KW - renal cortex and medulla
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U2 - 10.1142/S1793545814500217
DO - 10.1142/S1793545814500217
M3 - Article
AN - SCOPUS:84901299889
SN - 1793-5458
VL - 7
JO - Journal of Innovative Optical Health Sciences
JF - Journal of Innovative Optical Health Sciences
IS - 3
M1 - 1450021
ER -