Tethering of Gly-Arg-Gly-Asp-Ser-Pro-Lys Peptides on Mg-Doped Hydroxyapatite

Alessandro Pistone; Daniela Iannazzo; Claudia Espro; Signorino Galvagno; Anna Tampieri; Monica Montesi; Silvia Panseri; Monica Sandri

doi:10.1016/J.ENG.2017.01.007

Tethering of Gly-Arg-Gly-Asp-Ser-Pro-Lys Peptides on Mg-Doped Hydroxyapatite

Alessandro Pistone, Daniela Iannazzo, Claudia Espro, Signorino Galvagno, Anna Tampieri, Monica Montesi, Silvia Panseri, Monica Sandri

Houston Methodist

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Stem cell homing, namely the recruitment of mesenchymal stem cells (MSCs) to injured tissues, is highly effective for bone regeneration in vivo. In order to explore whether the incorporation of mimetic peptide sequences on magnesium-doped (Mg-doped) hydroxyapatite (HA) may regulate the homing of MSCs, and thus induce cell migration to a specific site, we covalently functionalized MgHA disks with two chemotactic/haptotactic factors: either the fibronectin fragment III₁-C human (FF III₁-C), or the peptide sequence Gly-Arg-Gly-Asp-Ser-Pro-Lys, a fibronectin analog that is able to bind to integrin transmembrane receptors. Preliminary biological evaluation of MSC viability, analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, suggested that stem cells migrate to the MgHA disks in response to the grafted haptotaxis stimuli.

Original language	English (US)
Pages (from-to)	55-59
Number of pages	5
Journal	Engineering
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/J.ENG.2017.01.007
State	Published - Feb 2017

Keywords

Bone tissue engineering
Chemotactic/haptotactic factors
Mesenchymal stem cells
Mg-doped hydroxyapatite

ASJC Scopus subject areas

General Computer Science
Environmental Engineering
General Chemical Engineering
Materials Science (miscellaneous)
Energy Engineering and Power Technology
General Engineering

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10.1016/J.ENG.2017.01.007

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title = "Tethering of Gly-Arg-Gly-Asp-Ser-Pro-Lys Peptides on Mg-Doped Hydroxyapatite",

abstract = "Stem cell homing, namely the recruitment of mesenchymal stem cells (MSCs) to injured tissues, is highly effective for bone regeneration in vivo. In order to explore whether the incorporation of mimetic peptide sequences on magnesium-doped (Mg-doped) hydroxyapatite (HA) may regulate the homing of MSCs, and thus induce cell migration to a specific site, we covalently functionalized MgHA disks with two chemotactic/haptotactic factors: either the fibronectin fragment III1-C human (FF III1-C), or the peptide sequence Gly-Arg-Gly-Asp-Ser-Pro-Lys, a fibronectin analog that is able to bind to integrin transmembrane receptors. Preliminary biological evaluation of MSC viability, analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, suggested that stem cells migrate to the MgHA disks in response to the grafted haptotaxis stimuli.",

keywords = "Bone tissue engineering, Chemotactic/haptotactic factors, Mesenchymal stem cells, Mg-doped hydroxyapatite",

author = "Alessandro Pistone and Daniela Iannazzo and Claudia Espro and Signorino Galvagno and Anna Tampieri and Monica Montesi and Silvia Panseri and Monica Sandri",

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year = "2017",

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AU - Pistone, Alessandro

AU - Iannazzo, Daniela

AU - Espro, Claudia

AU - Galvagno, Signorino

AU - Tampieri, Anna

AU - Montesi, Monica

AU - Panseri, Silvia

AU - Sandri, Monica

PY - 2017/2

Y1 - 2017/2

N2 - Stem cell homing, namely the recruitment of mesenchymal stem cells (MSCs) to injured tissues, is highly effective for bone regeneration in vivo. In order to explore whether the incorporation of mimetic peptide sequences on magnesium-doped (Mg-doped) hydroxyapatite (HA) may regulate the homing of MSCs, and thus induce cell migration to a specific site, we covalently functionalized MgHA disks with two chemotactic/haptotactic factors: either the fibronectin fragment III1-C human (FF III1-C), or the peptide sequence Gly-Arg-Gly-Asp-Ser-Pro-Lys, a fibronectin analog that is able to bind to integrin transmembrane receptors. Preliminary biological evaluation of MSC viability, analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, suggested that stem cells migrate to the MgHA disks in response to the grafted haptotaxis stimuli.

AB - Stem cell homing, namely the recruitment of mesenchymal stem cells (MSCs) to injured tissues, is highly effective for bone regeneration in vivo. In order to explore whether the incorporation of mimetic peptide sequences on magnesium-doped (Mg-doped) hydroxyapatite (HA) may regulate the homing of MSCs, and thus induce cell migration to a specific site, we covalently functionalized MgHA disks with two chemotactic/haptotactic factors: either the fibronectin fragment III1-C human (FF III1-C), or the peptide sequence Gly-Arg-Gly-Asp-Ser-Pro-Lys, a fibronectin analog that is able to bind to integrin transmembrane receptors. Preliminary biological evaluation of MSC viability, analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, suggested that stem cells migrate to the MgHA disks in response to the grafted haptotaxis stimuli.

KW - Bone tissue engineering

KW - Chemotactic/haptotactic factors

KW - Mesenchymal stem cells

KW - Mg-doped hydroxyapatite

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Tethering of Gly-Arg-Gly-Asp-Ser-Pro-Lys Peptides on Mg-Doped Hydroxyapatite

Abstract

Keywords

ASJC Scopus subject areas

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