Tethering of Gly-Arg-Gly-Asp-Ser-Pro-Lys Peptides on Mg-Doped Hydroxyapatite

Alessandro Pistone, Daniela Iannazzo, Claudia Espro, Signorino Galvagno, Anna Tampieri, Monica Montesi, Silvia Panseri, Monica Sandri

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Stem cell homing, namely the recruitment of mesenchymal stem cells (MSCs) to injured tissues, is highly effective for bone regeneration in vivo. In order to explore whether the incorporation of mimetic peptide sequences on magnesium-doped (Mg-doped) hydroxyapatite (HA) may regulate the homing of MSCs, and thus induce cell migration to a specific site, we covalently functionalized MgHA disks with two chemotactic/haptotactic factors: either the fibronectin fragment III1-C human (FF III1-C), or the peptide sequence Gly-Arg-Gly-Asp-Ser-Pro-Lys, a fibronectin analog that is able to bind to integrin transmembrane receptors. Preliminary biological evaluation of MSC viability, analyzed by 3-(4,5-dimethyl­thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, suggested that stem cells migrate to the MgHA disks in response to the grafted haptotaxis stimuli.

Original languageEnglish (US)
Pages (from-to)55-59
Number of pages5
JournalEngineering
Volume3
Issue number1
DOIs
StatePublished - Feb 2017

Keywords

  • Bone tissue engineering
  • Chemotactic/haptotactic factors
  • Mesenchymal stem cells
  • Mg-doped hydroxyapatite

ASJC Scopus subject areas

  • Computer Science(all)
  • Environmental Engineering
  • Chemical Engineering(all)
  • Materials Science (miscellaneous)
  • Energy Engineering and Power Technology
  • Engineering(all)

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