Abstract
Mutants of helper phage M13 KO7 were isolated whose survival was up to 300-fold greater than the wild type after 30 min at 95°C, and had increased solvent tolerance (up to 200-fold in 75% v/v N,N-dimethylformamide). These mutants retained their ability to package pIII-fusion display phagemids with normal efficiency and therefore will be useful in the isolation of stable protein variants by phage display.
Original language | English (US) |
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Pages (from-to) | 87-90 |
Number of pages | 4 |
Journal | Biotechnology Letters |
Volume | 21 |
Issue number | 1 |
DOIs | |
State | Published - 1999 |
Keywords
- M13
- Phage display
- Solvent tolerance
- Temperature resistance
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Microbiology