Temperature- and solvent-tolerant mutants of filamentous bacteriophage helper M13 KO7

Robert R. Riddle; Richard C. Willson; Michael J. Benedik

doi:10.1023/A:1005402209276

Temperature- and solvent-tolerant mutants of filamentous bacteriophage helper M13 KO7

Robert R. Riddle, Richard C. Willson, Michael J. Benedik

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Mutants of helper phage M13 KO7 were isolated whose survival was up to 300-fold greater than the wild type after 30 min at 95°C, and had increased solvent tolerance (up to 200-fold in 75% v/v N,N-dimethylformamide). These mutants retained their ability to package pIII-fusion display phagemids with normal efficiency and therefore will be useful in the isolation of stable protein variants by phage display.

Original language	English (US)
Pages (from-to)	87-90
Number of pages	4
Journal	Biotechnology Letters
Volume	21
Issue number	1
DOIs	https://doi.org/10.1023/A:1005402209276
State	Published - 1999

Keywords

M13
Phage display
Solvent tolerance
Temperature resistance

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Microbiology

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10.1023/A:1005402209276

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title = "Temperature- and solvent-tolerant mutants of filamentous bacteriophage helper M13 KO7",

abstract = "Mutants of helper phage M13 KO7 were isolated whose survival was up to 300-fold greater than the wild type after 30 min at 95°C, and had increased solvent tolerance (up to 200-fold in 75% v/v N,N-dimethylformamide). These mutants retained their ability to package pIII-fusion display phagemids with normal efficiency and therefore will be useful in the isolation of stable protein variants by phage display.",

keywords = "M13, Phage display, Solvent tolerance, Temperature resistance",

author = "Riddle, {Robert R.} and Willson, {Richard C.} and Benedik, {Michael J.}",

note = "Funding Information: We thank Ulrich Strych, Dakshina Jandhyala and Baby Djojonegoro for helpful discussions. This work was supported by the Robert A. Welch Foundation and by grants 3652-117, 3652-233 and 3652-272 from the Texas Advanced Research Program and PEER grant #06 from the University of Houston. Copyright: Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.",

year = "1999",

doi = "10.1023/A:1005402209276",

language = "English (US)",

volume = "21",

pages = "87--90",

journal = "Biotechnology Letters",

issn = "0141-5492",

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T1 - Temperature- and solvent-tolerant mutants of filamentous bacteriophage helper M13 KO7

AU - Riddle, Robert R.

AU - Willson, Richard C.

AU - Benedik, Michael J.

N1 - Funding Information: We thank Ulrich Strych, Dakshina Jandhyala and Baby Djojonegoro for helpful discussions. This work was supported by the Robert A. Welch Foundation and by grants 3652-117, 3652-233 and 3652-272 from the Texas Advanced Research Program and PEER grant #06 from the University of Houston. Copyright: Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.

PY - 1999

Y1 - 1999

N2 - Mutants of helper phage M13 KO7 were isolated whose survival was up to 300-fold greater than the wild type after 30 min at 95°C, and had increased solvent tolerance (up to 200-fold in 75% v/v N,N-dimethylformamide). These mutants retained their ability to package pIII-fusion display phagemids with normal efficiency and therefore will be useful in the isolation of stable protein variants by phage display.

AB - Mutants of helper phage M13 KO7 were isolated whose survival was up to 300-fold greater than the wild type after 30 min at 95°C, and had increased solvent tolerance (up to 200-fold in 75% v/v N,N-dimethylformamide). These mutants retained their ability to package pIII-fusion display phagemids with normal efficiency and therefore will be useful in the isolation of stable protein variants by phage display.

KW - M13

KW - Phage display

KW - Solvent tolerance

KW - Temperature resistance

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Temperature- and solvent-tolerant mutants of filamentous bacteriophage helper M13 KO7

Abstract

Keywords

ASJC Scopus subject areas

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