Temperature- and solvent-tolerant mutants of filamentous bacteriophage helper M13 KO7

Robert R. Riddle, Richard C. Willson, Michael J. Benedik

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Mutants of helper phage M13 KO7 were isolated whose survival was up to 300-fold greater than the wild type after 30 min at 95°C, and had increased solvent tolerance (up to 200-fold in 75% v/v N,N-dimethylformamide). These mutants retained their ability to package pIII-fusion display phagemids with normal efficiency and therefore will be useful in the isolation of stable protein variants by phage display.

Original languageEnglish (US)
Pages (from-to)87-90
Number of pages4
JournalBiotechnology Letters
Volume21
Issue number1
DOIs
StatePublished - 1999

Keywords

  • M13
  • Phage display
  • Solvent tolerance
  • Temperature resistance

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

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